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Appl Environ Microbiol. 1992 April; 58(4): 1376-1378

Conditions for protoplasting, regenerating, and transforming the calicheamicin producer, Micromonospora echinospora.

Department of Microbial Genetics, Lederle Laboratories, Pearl River, New York 10965.

ABSTRACT

We report methods to generate protoplasts, to regenerate mycelia, and to transform Micromonospora echinospora. This actinomycete produces the unusual antitumor antibiotics, the calicheamicins. These protocols may be applied to other actinomycetes that have been difficult to transform. These methods also may facilitate the cloning of calicheamicin biosynthetic genes by genetic complementation.

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