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Appl Environ Microbiol. 1992 April; 58(4): 1402-1407

Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52.

Department of Food Science, Pennsylvania State University, University Park 16802.

ABSTRACT

In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50,241. The predicted enzyme was found to contain an amino acid sequence highly homologous to the putative substrate-binding domain present within all lipases examined to date.

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