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Appl Environ Microbiol. 1992 April; 58(4): 1411-1414

Sporulation and enterotoxin production by Clostridium perfringens type A at 37 and 43 degrees C.

Departamento de Microbiologia, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico.

ABSTRACT

Enterotoxin-positive strains of Clostridium perfringens were grown in Duncan-Strong sporulation medium in the presence of 0.4% (7.9 mM) raffinose at 37 and 43 degrees C. Enterotoxin- and heat-resistant spores were produced at similar concentrations but sooner at 43 degrees C than at 37 degrees C. There was a direct relationship between spore heat resistance and sporulation temperature (32, 37, and 43 degrees C).

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