This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stoddard, G W Articles by McKay, L L Search for Related Content PubMed PubMed Citation Articles by Stoddard, G W Articles by McKay, L L Agricola Articles by Stoddard, G W Articles by McKay, L L

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1992 June; 58(6): 1952-1961

Molecular analyses of the lactococcin A gene cluster from Lactococcus lactis subsp. lactis biovar diacetylactis WM4.

Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108.

ABSTRACT

The genes responsible for bacteriocin production and immunity in Lactococcus lactis subsp. lactis biovar diacetylactis WM4 were localized and characterized by DNA restriction fragment deletion, subcloning, and nucleotide sequence analysis. The nucleotide sequence of a 5.6-kb AvaII restriction fragment revealed a cluster with five complete open reading frames (ORFs) in the same orientation. DNA and protein homology analyses, combined with deletion and Tn5 insertion mutagenesis, implicated four of the ORFs in the production of and immunity to lactococcin A. The last two ORFs in the cluster were the lactococcin A structural and immunity genes, lcnA and lciA. The two ORFs immediately upstream of lcnA and lciA were designated lcnC and lcnD, and the proteins that they encoded showed similarities to proteins of signal sequence-independent secretion systems. lcnC encodes a protein of 716 amino acids that could belong to the HlyB family of ATP-dependent membrane translocators. LcnC contains an ATP binding domain in a conserved C-terminal stretch of approximately 200 amino acids and three putative hydrophobic segments in the N terminus. The lcnD product, LcnD, of 474 amino acids, is essential for lactococcin A expression and shows structural similarities to HlyD and its homologs. On the basis of these results, a secretion apparatus that is essential for the full expression of active lactococcin A is postulated.

This article has been cited by other articles:

• Fontaine, L., Boutry, C., Guedon, E., Guillot, A., Ibrahim, M., Grossiord, B., Hols, P. (2007). Quorum-Sensing Regulation of the Production of Blp Bacteriocins in Streptococcus thermophilus. J. Bacteriol. 189: 7195-7205 [Abstract] [Full Text]  
• Diep, D. B., Skaugen, M., Salehian, Z., Holo, H., Nes, I. F. (2007). Common mechanisms of target cell recognition and immunity for class II bacteriocins. Proc. Natl. Acad. Sci. USA 104: 2384-2389 [Abstract] [Full Text]  
• Diep, D. B., Godager, L., Brede, D., Nes, I. F. (2006). Data mining and characterization of a novel pediocin-like bacteriocin system from the genome of Pediococcus pentosaceus ATCC 25745. Microbiology 152: 1649-1659 [Abstract] [Full Text]  
• Kemperman, R., Jonker, M., Nauta, A., Kuipers, O. P., Kok, J. (2003). Functional Analysis of the Gene Cluster Involved in Production of the Bacteriocin Circularin A by Clostridium beijerinckii ATCC 25752. Appl. Environ. Microbiol. 69: 5839-5848 [Abstract] [Full Text]  
• Gajic, O., Buist, G., Kojic, M., Topisirovic, L., Kuipers, O. P., Kok, J. (2003). Novel Mechanism of Bacteriocin Secretion and Immunity Carried Out by Lactococcal Multidrug Resistance Proteins. J. Biol. Chem. 278: 34291-34298 [Abstract] [Full Text]  
• Dorenbos, R., Stein, T., Kabel, J., Bruand, C., Bolhuis, A., Bron, S., Quax, W. J., van Dijl, J. M. (2002). Thiol-Disulfide Oxidoreductases Are Essential for the Production of the Lantibiotic Sublancin 168. J. Biol. Chem. 277: 16682-16688 [Abstract] [Full Text]  
• Turner, M. S., Helmann, J. D. (2000). Mutations in Multidrug Efflux Homologs, Sugar Isomerases, and Antimicrobial Biosynthesis Genes Differentially Elevate Activity of the sigma X and sigma W Factors in Bacillus subtilis. J. Bacteriol. 182: 5202-5210 [Abstract] [Full Text]  
• Martínez, J. M., Kok, J., Sanders, J. W., Hernández, P. E. (2000). Heterologous Coproduction of Enterocin A and Pediocin PA-1 by Lactococcus lactis: Detection by Specific Peptide-Directed Antibodies. Appl. Environ. Microbiol. 66: 3543-3549 [Abstract] [Full Text]  
• Horn, N., Martínez, M. I., Martínez, J. M., Hernández, P. E., Gasson, M. J., Rodríguez, J. M., Dodd, H. M. (1999). Enhanced Production of Pediocin PA-1 and Coproduction of Nisin and Pediocin PA-1 by Lactococcus lactis. Appl. Environ. Microbiol. 65: 4443-4450 [Abstract] [Full Text]  
• Franke, C. M., Tiemersma, J., Venema, G., Kok, J. (1999). Membrane Topology of the Lactococcal Bacteriocin ATP-binding Cassette Transporter Protein LcnC. INVOLVEMENT OF LcnC IN LACTOCOCCIN A MATURATION. J. Biol. Chem. 274: 8484-8490 [Abstract] [Full Text]  
• McCormick, J. K., Poon, A., Sailer, M., Gao, Y., Roy, K. L., McMullen, L. M., Vederas, J. C., Stiles, M. E., Van Belkum, M. J. (1998). Genetic Characterization and Heterologous Expression of Brochocin-C, an Antibotulinal, Two-Peptide Bacteriocin Produced by Brochothrix campestris ATCC 43754. Appl. Environ. Microbiol. 64: 4757-4766 [Abstract] [Full Text]  
• Horn, N., Martínez, M. I., Martínez, J. M., Hernández, P. E., Gasson, M. J., Rodríguez, J. M., Dodd, H. M. (1998). Production of Pediocin PA-1 by Lactococcus lactis Using the Lactococcin A Secretory Apparatus. Appl. Environ. Microbiol. 64: 818-823 [Abstract] [Full Text]  
• Fleury, Y., Dayem, M. A., Montagne, J. J., Chaboisseau, E., Caer, J. P. L., Nicolas, P., Delfour, A. (1996). Covalent Structure, Synthesis, and Structure-Function Studies of Mesentericin Y 10537, a Defensive Peptide from Gram-positive Bacteria Leuconostoc mesenteroides. J. Biol. Chem. 271: 14421-14429 [Abstract] [Full Text]