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Next Article 

Appl Environ Microbiol. 1993 December; 59(12): 3985-3988

Nutritional aspects of cytotoxin production by Clostridium difficile.

D P Osgood, N P Wood and J F Sperry

Department of Microbiology, University of Rhode Island, Kingston 02881.

ABSTRACT

Arginine was the only amino acid used by Clostridium difficile that permitted cytotoxin synthesis in a peptone-based medium. Synthesis of cytotoxin was delayed when glucose was used as the substrate. Addition of rifampin or puromycin to cultures prior to release of cytotoxin inhibited the release of cytotoxin, suggesting that a protein essential for cytotoxin release is synthesized after cytotoxin is synthesized.

Appl Environ Microbiol. 1993 December; 59(12): 3985-3988




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