This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Finch, G R
Right arrow Articles by Belosevic, M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Finch, G R
Right arrow Articles by Belosevic, M
Agricola
Right arrow Articles by Finch, G R
Right arrow Articles by Belosevic, M

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1993 December; 59(12): 4203-4210

Ozone inactivation of Cryptosporidium parvum in demand-free phosphate buffer determined by in vitro excystation and animal infectivity.

G R Finch, E K Black, L Gyürék and M Belosevic

Department of Civil Engineering, University of Alberta, Edmonton, Canada.

ABSTRACT

Inactivation of Cryptosporidium parvum oocysts by ozone was performed in ozone demand-free 0.05 M phosphate buffer (pH 6.9) in bench-scale batch reactors at 7 and 22 degrees C. Ozone was added to each trial from a concentrated stock solution for contact times ranging from 5 to 15 min. The viability of the control and treated oocysts was determined by using in vitro excystation and infection in neonatal CD-1 mice. It was found that excystation consistently underestimated inactivation when compared with animal infectivity (P < or = 0.05). As inactivations increased, the difference between excystation and infectivity also increased. The inactivation kinetics of C. parvum by ozone deviated from the simple first-order Chick-Watson model and was better described by a nonlinear Hom model. The use of the Hom model for predicting inactivation resulted in a family of unique concentration and time values for each inactivation level rather than the simple CT product of the Chick-Watson model.

Appl Environ Microbiol. 1993 December; 59(12): 4203-4210




This article has been cited by other articles:

  • Brescia, C. C., Griffin, S. M., Ware, M. W., Varughese, E. A., Egorov, A. I., Villegas, E. N. (2009). Cryptosporidium Propidium Monoazide-PCR, a Molecular Biology-Based Technique for Genotyping of Viable Cryptosporidium Oocysts. Appl. Environ. Microbiol. 75: 6856-6863 [Abstract] [Full Text]  
  • King, B. J., Keegan, A. R., Monis, P. T., Saint, C. P. (2005). Environmental Temperature Controls Cryptosporidium Oocyst Metabolic Rate and Associated Retention of Infectivity. Appl. Environ. Microbiol. 71: 3848-3857 [Abstract] [Full Text]  
  • Quilez, J., Sanchez-Acedo, C., Avendano, C., del Cacho, E., Lopez-Bernad, F. (2005). Efficacy of Two Peroxygen-Based Disinfectants for Inactivation of Cryptosporidium parvum Oocysts. Appl. Environ. Microbiol. 71: 2479-2483 [Abstract] [Full Text]  
  • Di Giovanni, G. D., LeChevallier, M. W. (2005). Quantitative-PCR Assessment of Cryptosporidium parvum Cell Culture Infection. Appl. Environ. Microbiol. 71: 1495-1500 [Abstract] [Full Text]  
  • Keegan, A. R., Fanok, S., Monis, P. T., Saint, C. P. (2003). Cell Culture-Taqman PCR Assay for Evaluation of Cryptosporidium parvum Disinfection. Appl. Environ. Microbiol. 69: 2505-2511 [Abstract] [Full Text]  
  • Cho, M., Chung, H., Yoon, J. (2003). Disinfection of Water Containing Natural Organic Matter by Using Ozone-Initiated Radical Reactions. Appl. Environ. Microbiol. 69: 2284-2291 [Abstract] [Full Text]  
  • Morita, S., Namikoshi, A., Hirata, T., Oguma, K., Katayama, H., Ohgaki, S., Motoyama, N., Fujiwara, M. (2002). Efficacy of UV Irradiation in Inactivating Cryptosporidiumparvum Oocysts. Appl. Environ. Microbiol. 68: 5387-5393 [Abstract] [Full Text]  
  • Chauret, C. P., Radziminski, C. Z., Lepuil, M., Creason, R., Andrews, R. C. (2001). Chlorine Dioxide Inactivation of Cryptosporidium parvum Oocysts and Bacterial Spore Indicators. Appl. Environ. Microbiol. 67: 2993-3001 [Abstract] [Full Text]  
  • Bukhari, Z., Marshall, M. M., Korich, D. G., Fricker, C. R., Smith, H. V., Rosen, J., Clancy, J. L. (2000). Comparison of Cryptosporidium parvum Viability and Infectivity Assays following Ozone Treatment of Oocysts. Appl. Environ. Microbiol. 66: 2972-2980 [Abstract] [Full Text]  
  • Neumann, N. F., Gyürek, L. L., Gammie, L., Finch, G. R., Belosevic, M. (2000). Comparison of Animal Infectivity and Nucleic Acid Staining for Assessment of Cryptosporidium parvum Viability in Water. Appl. Environ. Microbiol. 66: 406-412 [Abstract] [Full Text]  
  • DuPont, H. L., Chappell, C. L., Sterling, C. R., Okhuysen, P. C., Rose, J. B., Jakubowski, W. (1995). The Infectivity of Cryptosporidium parvum in Healthy Volunteers. NEJM 332: 855-859 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»