AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Recorbet, G
Right arrow Articles by Simonet, P
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Recorbet, G
Right arrow Articles by Simonet, P
Agricola
Right arrow Articles by Recorbet, G
Right arrow Articles by Simonet, P

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1993 December; 59(12): 4289-4294

Kinetics of the persistence of chromosomal DNA from genetically engineered Escherichia coli introduced into soil.

G Recorbet, C Picard, P Normand and P Simonet

Laboratoire d'Ecologie Microbienne du Sol, URA Centre National de la Recherche Scientifique 1450, Université Lyon I, Villeurbanne, France.

ABSTRACT

Investigations to quantify bacterial survival and DNA persistence of a genetically engineered population of Escherichia coli introduced into soil microcosms were carried out. The survival of E. coli was monitored by plate counting and immunofluorescence methods, whereas the persistence of the DNA was evaluated by using a most-probable-number-polymerase chain reaction method. Whereas the E. coli population density declined below the plate-counting-technique detection threshold (10(2) CFU.g-1) after 15 days, 10(3) extracellular and 5 x 10(5) total DNA target sequences were still detected after 40 days. Additionally, the E. coli cell counts fell below the detection limit of the immunofluorescence method (10(5) cells.g-1) before the end of the experiment. Colony hybridizations did not reveal gene transfer to the indigenous microflora. These results confirm the persistence of residual E. coli target sequences that could not be detected by the classical cell counting method and offer promising applications for the environmental detection of microorganisms, either engineered, pathogenic, or released for beneficial effects.

Appl Environ Microbiol. 1993 December; 59(12): 4289-4294




This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1993 by the American Society for Microbiology. All rights reserved.