This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by van der Waarde, J J Articles by Janssen, D B Search for Related Content PubMed PubMed Citation Articles by van der Waarde, J J Articles by Janssen, D B Agricola Articles by van der Waarde, J J Articles by Janssen, D B

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1993 February; 59(2): 528-535

Degradation of 2-chloroallylalcohol by a Pseudomonas sp.

Department of Biochemistry, University of Groningen, The Netherlands.

ABSTRACT

Three Pseudomonas strains capable of utilizing 2-chloroallylalcohol (2-chloropropenol) as the sole carbon source for growth were isolated from soil. The fastest growth was observed with strain JD2, with a generation time of 3.6 h. Degradation of 2-chloroallylalcohol was accompanied by complete dehalogenation. Chloroallylalcohols that did not support growth were dechlorinated by resting cells; the dechlorination level was highest if an alpha-chlorine substituent was present. Crude extracts of strain JD2 contained inducible alcohol dehydrogenase activity that oxidized mono- and dichloroallylalcohols but not trichloroallylalcohol. The enzyme used phenazine methosulfate as an artificial electron acceptor. Further oxidation yielded 2-chloroacrylic acid. The organism also produced hydrolytic dehalogenases converting 2-chloroacetic acid and 2-chloropropionic acid.

This article has been cited by other articles:

• Kurata, A., Kurihara, T., Kamachi, H., Esaki, N. (2005). 2-Haloacrylate Reductase, a Novel Enzyme of the Medium Chain Dehydrogenase/Reductase Superfamily That Catalyzes the Reduction of a Carbon-Carbon Double Bond of Unsaturated Organohalogen Compounds. J. Biol. Chem. 280: 20286-20291 [Abstract] [Full Text]  
• Dungan, R. S., Yates, S. R. (2003). Degradation of Fumigant Pesticides: 1,3-Dichloropropene, Methyl Isothiocyanate, Chloropicrin, and Methyl Bromide. Vadose Zone J 2: 279-286 [Abstract] [Full Text]  
• Poelarends, G. J., Kulakov, L. A., Larkin, M. J., van Hylckama Vlieg, J. E. T., Janssen, D. B. (2000). Roles of Horizontal Gene Transfer and Gene Integration in Evolution of 1,3-Dichloropropene- and 1,2-Dibromoethane-Degradative Pathways. J. Bacteriol. 182: 2191-2199 [Abstract] [Full Text]  
• Poelarends, G. J., Wilkens, M., Larkin, M. J., van Elsas, J. D., Janssen, D. B. (1998). Degradation of 1,3-Dichloropropene by Pseudomonas cichorii 170. Appl. Environ. Microbiol. 64: 2931-2936 [Abstract] [Full Text]