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 Previous Article

Appl Environ Microbiol. 1993 April; 59(4): 1253-1258

Expression and secretion of Bacillus amyloliquefaciens alpha-amylase by using the yeast pheromone alpha-factor promoter and leader sequence in Saccharomyces cerevisiae.

V J Southgate, A J Steyn, I S Pretorius and H J Van Vuuren

Department of Microbiology, University of Stellenbosch, South Africa.

ABSTRACT

Replacement of the regulatory and secretory signals of the alpha-amylase gene (AMY) from Bacillus amylolique-faciens with the complete yeast pheromone alpha-factor prepro region (MF alpha 1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala)2 peptide from the MF alpha 1 spacer region (Lys-Arg-Glu-Ala-Glu-Ala) yielded decreased levels of extracellular alpha-amylase.

Appl Environ Microbiol. 1993 April; 59(4): 1253-1258






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