This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, C Articles by Dawson, K A Search for Related Content PubMed PubMed Citation Articles by Lee, C Articles by Dawson, K A Agricola Articles by Lee, C Articles by Dawson, K A

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1993 May; 59(5): 1467-1472

Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure.

Department of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China.

ABSTRACT

A total of 114 gram-negative fecal isolates from domestic pigs in herds with different histories of antimicrobial agent exposure were screened for the presence of plasmid DNA and specific tetracycline resistance determinants. More than 84% of the isolates harbored plasmid DNA, which ranged in size from 2.1 to 186 kb. A total of 78 isolates (68.4%) were resistant to tetracycline at concentrations greater than 4 micrograms/ml. Plasmid DNAs from about 56% of the tetracycline-resistant isolates hybridized with DNA probes for class A, B, C, and D tetracycline resistance determinants. The class B determinant was the most common determinant (35% of the isolates), followed by the class C determinant (12%) and the class A determinant (1%). About 9% of the isolates contained two determinants on plasmids. None of the plasmids from isolates hybridized with the class D determinant probe. The class C determinant was the most prevalent determinant on plasmids in isolates from pigs not exposed to antimicrobial agents for more than 146 months, while the class B determinant was more prevalent on plasmids in isolates from pigs exposed to either subtherapeutic or therapeutic levels of antimicrobial agents. Most tetracycline resistance determinants were localized on plasmids which were more than 30 kb long. A great number of wild-type tetracycline-resistant Escherichia coli strains were found with the class E determinant on their chromosomes. This study revealed a high prevalence of tetracycline resistance determinants in the fecal flora of pig herds whether or not they were fed with antibiotics.

This article has been cited by other articles:

• Sharma, R., Munns, K., Alexander, T., Entz, T., Mirzaagha, P., Yanke, L. J., Mulvey, M., Topp, E., McAllister, T. (2008). Diversity and Distribution of Commensal Fecal Escherichia coli Bacteria in Beef Cattle Administered Selected Subtherapeutic Antimicrobials in a Feedlot Setting. Appl. Environ. Microbiol. 74: 6178-6186 [Abstract] [Full Text]  
• Thompson, S. A., Maani, E. V., Lindell, A. H., King, C. J., McArthur, J. V. (2007). Novel Tetracycline Resistance Determinant Isolated from an Environmental Strain of Serratia marcescens. Appl. Environ. Microbiol. 73: 2199-2206 [Abstract] [Full Text]  
• Sawant, A. A., Hegde, N. V., Straley, B. A., Donaldson, S. C., Love, B. C., Knabel, S. J., Jayarao, B. M. (2007). Antimicrobial-Resistant Enteric Bacteria from Dairy Cattle. Appl. Environ. Microbiol. 73: 156-163 [Abstract] [Full Text]  
• Khachatryan, A. R., Besser, T. E., Hancock, D. D., Call, D. R. (2006). Use of a Nonmedicated Dietary Supplement Correlates with Increased Prevalence of Streptomycin-Sulfa-Tetracycline-Resistant Escherichia coli on a Dairy Farm.. Appl. Environ. Microbiol. 72: 4583-4588 [Abstract] [Full Text]  
• Hoyle, D. V., Yates, C. M., Chase-Topping, M. E., Turner, E. J., Davies, S. E., Low, J. C., Gunn, G. J., Woolhouse, M. E. J., Amyes, S. G. B. (2005). Molecular Epidemiology of Antimicrobial-Resistant Commensal Escherichia coli Strains in a Cohort of Newborn Calves. Appl. Environ. Microbiol. 71: 6680-6688 [Abstract] [Full Text]  
• Maynard, C., Fairbrother, J. M., Bekal, S., Sanschagrin, F., Levesque, R. C., Brousseau, R., Masson, L., Lariviere, S., Harel, J. (2003). Antimicrobial Resistance Genes in Enterotoxigenic Escherichia coli O149:K91 Isolates Obtained over a 23-Year Period from Pigs. Antimicrob. Agents Chemother. 47: 3214-3221 [Abstract] [Full Text]  
• Hartman, A. B., Essiet, I. I., Isenbarger, D. W., Lindler, L. E. (2003). Epidemiology of Tetracycline Resistance Determinants in Shigella spp. and Enteroinvasive Escherichia coli: Characterization and Dissemination of tet(A)-1. J. Clin. Microbiol. 41: 1023-1032 [Abstract] [Full Text]  
• Luber, P., Bartelt, E., Genschow, E., Wagner, J., Hahn, H. (2003). Comparison of Broth Microdilution, E Test, and Agar Dilution Methods for Antibiotic Susceptibility Testing of Campylobacter jejuni and Campylobacter coli. J. Clin. Microbiol. 41: 1062-1068 [Abstract] [Full Text]  
• Fluit, A. C., Visser, M. R., Schmitz, F.-J. (2001). Molecular Detection of Antimicrobial Resistance. Clin. Microbiol. Rev. 14: 836-871 [Abstract] [Full Text]  
• Chopra, I., Roberts, M. (2001). Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance. Microbiol. Mol. Biol. Rev. 65: 232-260 [Abstract] [Full Text]  
• Chambers, P. A., Duggan, P. S., Forbes, J. M., Heritage, J. (2001). A rapid, reliable method for the extraction from avian faeces of total bacterial DNA to be used as a template for the detection of antibiotic resistance genes. J Antimicrob Chemother 47: 241-243 [Full Text]  
• Rhodes, G., Huys, G., Swings, J., Mcgann, P., Hiney, M., Smith, P., Pickup, R. W. (2000). Distribution of Oxytetracycline Resistance Plasmids between Aeromonads in Hospital and Aquaculture Environments: Implication of Tn1721 in Dissemination of the Tetracycline Resistance Determinant Tet A. Appl. Environ. Microbiol. 66: 3883-3890 [Abstract] [Full Text]