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Appl Environ Microbiol. 1994 January; 60(1): 323-327

A method for detection of aromatic metabolites at very low concentrations: application to detection of metabolites of anaerobic toluene degradation.

E A Edwards, A M Edwards and D Grbi-Gali

Department of Civil Engineering, Standford University, California 94305-4020.

ABSTRACT

Difficulties inherent in working with anaerobic microorganisms and mixed cultures have hampered efforts to detect and identify metabolites of anaerobic degradation of monoaromatic compounds. Isotope-trapping experiments and analysis using a high-performance liquid chromatograph equipped with a flow-through radioactivity detector were used to detect very low concentrations of metabolites. Data obtained by this method suggest that toluene was degraded via methyl hydroxylation by a mixed methanogenic culture.

This article has been cited by other articles:

• Beller, H. R., Edwards, E. A. (2000). Anaerobic Toluene Activation by Benzylsuccinate Synthase in a Highly Enriched Methanogenic Culture. Appl. Environ. Microbiol. 66: 5503-5505 [Abstract] [Full Text]  
• Ficker, M., Krastel, K., Orlicky, S., Edwards, E. (1999). Molecular Characterization of a Toluene-Degrading Methanogenic Consortium. Appl. Environ. Microbiol. 65: 5576-5585 [Abstract] [Full Text]