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Appl Environ Microbiol. 1994 November; 60(11): 4199-4202

Expression of mosquitocidal toxin genes in a gas-vacuolated strain of Ancylobacter aquaticus.

Institute of Molecular and Cell Biology, National University of Singapore.

ABSTRACT

A series of plasmids bearing the binary toxin genes of Bacillus sphaericus 2297 or 2317.3, the 100-kDa toxin gene of B. sphaericus SSII-1, or the 130-kDa (cryIVB) toxin gene of Bacillus thuringiensis subsp. israelensis were constructed and introduced into Ancylobacter aquaticus by electroporation. The transformed A. aquaticus cells exhibited significant toxicity towards mosquito larvae, demonstrating a potential use of recombinant A. aquaticus for biological control of mosquitoes.

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