This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mills, D A Articles by McKay, L L Search for Related Content PubMed PubMed Citation Articles by Mills, D A Articles by McKay, L L Agricola Articles by Mills, D A Articles by McKay, L L

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1994 December; 60(12): 4413-4420

Genetic analysis of regions of the Lactococcus lactis subsp. lactis plasmid pRS01 involved in conjugative transfer.

Department of Microbiology, University of Minnesota, St. Paul 55108.

ABSTRACT

The genes responsible for conjugative transfer of the 48.4-kb Lactococcus lactis subsp. lactis ML3 plasmid pRS01 were localized by insertional mutagenesis. Integration of the IS946-containing plasmid pTRK28 into pRS01 generated a pool of stable cointegrates, including a number of plasmids altered in conjugative proficiency. Mapping of pTRK28 insertions and phenotypic analysis of cointegrate plasmids identified four distinct regions (Tra1, Tra2, Tra3, and Tra4) involved in pRS01 conjugative transfer. Tra3 corresponds closely to a region previously identified (D. G. Anderson and L. L. McKay, J. Bacteriol. 158:954-962, 1984). Another region (Tra4) was localized within an inversion sequence shown to correlate with a cell aggregation phenotype. Tra1 and Tra2, two previously unidentified regions, were located at a distance of 9 kb from Tra3. When provided in trans, a cloned portion of the Tra3 region complemented Tra3 mutants.

This article has been cited by other articles:

• Belhocine, K., Mak, A. B., Cousineau, B. (2007). Trans-splicing of the Ll.LtrB group II intron in Lactococcus lactis. Nucleic Acids Res 35: 2257-2268 [Abstract] [Full Text]  
• Slagter-Jager, J. G., Allen, G. S., Smith, D., Hahn, I. A., Frank, J., Belfort, M. (2006). Visualization of a group II intron in the 23S rRNA of a stable ribosome. Proc. Natl. Acad. Sci. USA 103: 9838-9843 [Abstract] [Full Text]  
• Chen, Y., Klein, J. R., McKay, L. L., Dunny, G. M. (2005). Quantitative Analysis of Group II Intron Expression and Splicing in Lactococcus lactis. Appl. Environ. Microbiol. 71: 2576-2586 [Abstract] [Full Text]  
• Klein, J. R., Chen, Y., Manias, D. A., Zhuo, J., Zhou, L., Peebles, C. L., Dunny, G. M. (2004). A Conjugation-Based System for Genetic Analysis of Group II Intron Splicing in Lactococcus lactis. J. Bacteriol. 186: 1991-1998 [Abstract] [Full Text]  
• Ichiyanagi, K., Beauregard, A., Belfort, M. (2003). A bacterial group II intron favors retrotransposition into plasmid targets. Proc. Natl. Acad. Sci. USA 100: 15742-15747 [Abstract] [Full Text]  
• Grohmann, E., Muth, G., Espinosa, M. (2003). Conjugative Plasmid Transfer in Gram-Positive Bacteria. Microbiol. Mol. Biol. Rev. 67: 277-301 [Abstract] [Full Text]  
• Frazier, C. L., San Filippo, J., Lambowitz, A. M., Mills, D. A. (2003). Genetic Manipulation of Lactococcus lactis by Using Targeted Group II Introns: Generation of Stable Insertions without Selection. Appl. Environ. Microbiol. 69: 1121-1128 [Abstract] [Full Text]  
• Antiporta, M. H., Dunny, G. M. (2002). ccfA, the Genetic Determinant for the cCF10 Peptide Pheromone in Enterococcus faecalis OG1RF. J. Bacteriol. 184: 1155-1162 [Abstract] [Full Text]  
• Hickey, R. M., Twomey, D. P., Ross, R. P., Hill, C. (2001). Exploitation of Plasmid pMRC01 To Direct Transfer of Mobilizable Plasmids into Commercial Lactococcal Starter Strains. Appl. Environ. Microbiol. 67: 2853-2858 [Abstract] [Full Text]  
• Le Bourgeois, P., Daveran-Mingot, M.-L., Ritzenthaler, P. (2000). Genome Plasticity among Related Lactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms. J. Bacteriol. 182: 2481-2491 [Abstract] [Full Text]  
• Mills, D. A., Phister, T. G., Dunny, G. M., McKay, L. L. (1998). An Origin of Transfer (oriT) on the Conjugative Element pRS01 from Lactococcus lactis subsp. lactis ML3. Appl. Environ. Microbiol. 64: 1541-1544 [Abstract] [Full Text]