This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by van Berkum, P Articles by Vargas, A A Search for Related Content PubMed PubMed Citation Articles by van Berkum, P Articles by Vargas, A A Agricola Articles by van Berkum, P Articles by Vargas, A A

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1994 February; 60(2): 554-561

Classification of the uptake hydrogenase-positive (Hup+) bean rhizobia as Rhizobium tropici.

Soybean and Alfalfa Research Laboratory, USDA Agricultural Research Service, Beltsville, Maryland 20705.

ABSTRACT

Phenotypic and genetic characterization indicated that Hup+ bean rhizobial strains are type IIA and type IIB Rhizobium tropici. The Hup+ strain USDA 2840, which did not cluster with either of the two types of R. tropici in a restriction fragment length polymorphism analysis, had electrophoretic patterns of PCR products generated with primers for repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus sequences similar to those of three reference strains of R. tropici type IIA. The Hup+ strain USDA 2738, which clustered with the reference strain of R. tropici IIB in a restriction fragment length polymorphism analysis, had electrophoretic patterns of PCR products generated with primers for repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus sequences more closely resembling those of the reference strains of R. tropici type IIA than those of type IIB. DNA amplification with the Y1 and Y2 primers to generate a portion of the 16S rDNA operon was useful to distinguish R. tropici type IIA strains from other bean rhizobial strains. The phylogenetic position of the type IIA strain of R. tropici USDA 2840, determined from the partial 16S rDNA sequence, indicated a more distant relationship with the type IIB strain of R. tropici CIAT899 than with the as yet unnamed rhizobial species of Leucaena leucocephala, TAL 1145. Therefore, we suggest that it may be appropriate either to separate R. tropici types IIA and IIB into two different species or to identify TAL 1145 to the species level as a third type of R. tropici.

This article has been cited by other articles:

• Baginsky, C., Brito, B., Imperial, J., Palacios, J.-M., Ruiz-Argueso, T. (2002). Diversity and Evolution of Hydrogenase Systems in Rhizobia. Appl. Environ. Microbiol. 68: 4915-4924 [Abstract] [Full Text]  
• Aguilar, O. M., López, M. V., Riccillo, P. M., González, R. A., Pagano, M., Grasso, D. H., Pühler, A., Favelukes, G. (1998). Prevalence of the Rhizobium etli-Like Allele in Genes Coding for 16S rRNA among the Indigenous Rhizobial Populations Found Associated with Wild Beans from the Southern Andes in Argentina. Appl. Environ. Microbiol. 64: 3520-3524 [Abstract] [Full Text]