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Appl Environ Microbiol. 1994 February; 60(2): 682-690
Copyright © 1994, American Society for Microbiology. All Rights Reserved.

Construction of a Bioinsecticidal Strain of Pseudomonas fluorescens Active against the Sugarcane Borer, Eldana saccharina

¹ FOODTEK, Council for Scientific and Industrial Research, Pretoria 0001, South Africa
² South African Sugar Association Experiment Station, Mount Edgecombe 4300, South Africa
³ Microbiology Department, University of Cape Town, Rondebosch 7700, South Africa

ABSTRACT

A cryIA(c) gene was cloned from a native Bacillus thuringiensis strain showing activity against the sugarcane borer, Eldana saccharina. The sequence of the cloned gene was very similar to that of the B. thuringiensis subsp. kurstaki HD-73 cryIA(c) gene. The gene was introduced into an isolate of Pseudomonas fluorescens, capable of colonizing sugarcane, on two broad-host-range plasmids, pDER405 and pKT240, having copy numbers of 13 and 28, respectively. By using the Omegon-Km vector, the cry gene was introduced into the chromosome of P. fluorescens isolate 14. Bioassays on eldana larvae showed that the strain carrying the gene integrated into the chromosome was as toxic as one carrying it on pKT240. Glasshouse trials indicated that sugarcane treated with P. fluorescens 14::Omegon-Km-cry were more resistant to eldana damage than untreated sugarcane was.

^* Corresponding author. Mailing address: Microbiology Dept., University of Cape Town, Rondebosch 7700, South Africa. Phone: 27 (21) 650 3269. Fax: 27 (21) 650 4023. Electronic mail address: JAT@ MICRO.UCT.AC.ZA.

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