This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Straub, T M Articles by Gerba, C P Search for Related Content PubMed PubMed Citation Articles by Straub, T M Articles by Gerba, C P Agricola Articles by Straub, T M Articles by Gerba, C P

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1994 March; 60(3): 1014-1017

A method to detect enteroviruses in sewage sludge-amended soil using the PCR.

Department of Soil and Water Science, University of Arizona, Tucson 85721.

ABSTRACT

PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.

This article has been cited by other articles:

• Thorne, C. M., Otvos, I. S., Conder, N., Levin, D. B. (2007). Development and Evaluation of Methods To Detect Nucleopolyhedroviruses in Larvae of the Douglas-Fir Tussock Moth, Orgyia pseudotsugata (McDunnough). Appl. Environ. Microbiol. 73: 1101-1106 [Abstract] [Full Text]  
• Shieh, Y.-S. C., Calci, K. R., Baric, R. S. (1999). A Method To Detect Low Levels of Enteric Viruses in Contaminated Oysters. Appl. Environ. Microbiol. 65: 4709-4714 [Abstract] [Full Text]  
• de Moraes, R. R., Maruniak, J. E., Funderburk, J. E. (1999). Methods for Detection of Anticarsia gemmatalis Nucleopolyhedrovirus DNA in Soil. Appl. Environ. Microbiol. 65: 2307-2311 [Abstract] [Full Text]