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Appl Environ Microbiol. 1994 March; 60(3): 921-926
Copyright © 1994, American Society for Microbiology. All Rights Reserved.

In Vitro Bleaching of Hardwood Kraft Pulp by Extracellular Enzymes Excreted from White Rot Fungi in a Cultivation System Using a Membrane Filter

¹ Department of Forest Products, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan

ABSTRACT

To clarify the role of excreted extracellular enzymes during long-term incubation in a pulp biobleaching system with white rot fungi, we developed a cultivation system in which a membrane filter is used; this membrane filter can prevent direct contact between hyphae and kraft pulp, but allows extracellular enzymes to attack the kraft pulp. Phanerochaete sordida YK-624 brightened the pulp 21.4 points to 54.0% brightness after a 5-day in vitro treatment; this value was significantly higher than the values obtained with Phanerochaete chrysosporium and Coriolus versicolor after a 7-day treatment. Our results indicate that cell-free, membrane-filtered components from the in vitro bleaching system are capable of delignifying unbleached kraft pulp. Obvious candidates for filterable reagents capable of delignifying and bleaching kraft pulp are peroxidase and phenoloxidase proteins. The level of secreted manganese peroxidase activity in the filterable components was substantial during strain YK-624 in vitro bleaching. A positive correlation between the level of manganese peroxidase and brightening of the pulp was observed.

^* Corresponding author. Mailing address: Department of Forest Products, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan 812. Phone: (92) 641-1101, ext. 6362. Fax: (92) 632-1956.

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