This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Goebel, B M Articles by Stackebrandt, E Search for Related Content PubMed PubMed Citation Articles by Goebel, B M Articles by Stackebrandt, E Agricola Articles by Goebel, B M Articles by Stackebrandt, E

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1994 May; 60(5): 1614-1621

Cultural and phylogenetic analysis of mixed microbial populations found in natural and commercial bioleaching environments.

Department of Microbiology, University of Queensland, St. Lucia, Australia.

ABSTRACT

A range of autotrophic and heterotrophic enrichment cultures were established to determine the cultural bacterial diversity present in samples obtained from the acidic runoff of a chalcocite overburden heap and from laboratory-scale (1- to 4-liter) batch and continuous bioreactors which were being used for the commercial assessment of the bioleachability of zinc sulfide ore concentrates. Strains identified as Thiobacillus ferrooxidans, Thiobacillus thiooxidans, "Leptospirillum ferrooxidans," and Acidiphilium cryptum were isolated from both the natural site and the batch bioreactor, but only "L. ferrooxidans," a moderately thermophilic strain of T. thiooxidans, and a moderately thermophilic iron-oxidizing bacterium could be recovered from the continuous bioreactor running under steady-state conditions. Sequence analysis of the 16S rRNA genes of 33 representative strains revealed that all of the strains were closely related to strains which have been sequenced previously and also confirmed the phylogenetic diversity of bacteria present in bioleaching environments.

This article has been cited by other articles:

• Ni, Y., Wan, D., He, K. (2008). 16S rDNA and 16S-23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Acidithiobacillus phylogeny. Microbiology 154: 2397-2407 [Abstract] [Full Text]  
• van Zyl, L. J., Deane, S. M., Louw, L.-A., Rawlings, D. E. (2008). Presence of a Family of Plasmids (29 to 65 Kilobases) with a 26-Kilobase Common Region in Different Strains of the Sulfur-Oxidizing Bacterium Acidithiobacillus caldus. Appl. Environ. Microbiol. 74: 4300-4308 [Abstract] [Full Text]  
• Hu, L., Pan, H., Xue, Y., Ventosa, A., Cowan, D. A., Jones, B. E., Grant, W. D., Ma, Y. (2008). Halorubrum luteum sp. nov., isolated from Lake Chagannor, Inner Mongolia, China. Int. J. Syst. Evol. Microbiol. 58: 1705-1708 [Abstract] [Full Text]  
• Mendez, M. O., Neilson, J. W., Maier, R. M. (2008). Characterization of a Bacterial Community in an Abandoned Semiarid Lead-Zinc Mine Tailing Site. Appl. Environ. Microbiol. 74: 3899-3907 [Abstract] [Full Text]  
• Blothe, M., Akob, D. M., Kostka, J. E., Goschel, K., Drake, H. L., Kusel, K. (2008). pH Gradient-Induced Heterogeneity of Fe(III)-Reducing Microorganisms in Coal Mining-Associated Lake Sediments. Appl. Environ. Microbiol. 74: 1019-1029 [Abstract] [Full Text]  
• Di Cagno, R., Buchin, S., de Candia, S., De Angelis, M., Fox, P. F., Gobbetti, M. (2007). Characterization of Italian Cheeses Ripened Under Nonconventional Conditions. J DAIRY SCI 90: 2689-2704 [Abstract] [Full Text]  
• Rawlings, D. E., Johnson, D. B. (2007). The microbiology of biomining: development and optimization of mineral-oxidizing microbial consortia. Microbiology 153: 315-324 [Abstract] [Full Text]  
• Kotze, A. A., Tuffin, I. M., Deane, S. M., Rawlings, D. E. (2006). Cloning and characterization of the chromosomal arsenic resistance genes from Acidithiobacillus caldus and enhanced arsenic resistance on conjugal transfer of ars genes located on transposon TnAtcArs. Microbiology 152: 3551-3560 [Abstract] [Full Text]  
• Coda, R., Brechany, E., De Angelis, M., De Candia, S., Di Cagno, R., Gobbetti, M. (2006). Comparison of the compositional, microbiological, biochemical, and volatile profile characteristics of nine Italian ewes' milk cheeses.. J DAIRY SCI 89: 4126-4143 [Abstract] [Full Text]  
• Nicomrat, D., Dick, W. A., Tuovinen, O. H. (2006). Microbial populations identified by fluorescence in situ hybridization in a constructed wetland treating Acid coal mine drainage.. J. Environ. Qual. 35: 1329-1337 [Abstract] [Full Text]  
• Rodriguez-Nava, V., Couble, A., Devulder, G., Flandrois, J.-P., Boiron, P., Laurent, F. (2006). Use of PCR-Restriction Enzyme Pattern Analysis and Sequencing Database for hsp65 Gene-Based Identification of Nocardia Species. J. Clin. Microbiol. 44: 536-546 [Abstract] [Full Text]  
• Elliott, D. R., Wilson, M., Buckley, C. M. F., Spratt, D. A. (2005). Cultivable Oral Microbiota of Domestic Dogs. J. Clin. Microbiol. 43: 5470-5476 [Abstract] [Full Text]  
• Hamamura, N., Olson, S. H., Ward, D. M., Inskeep, W. P. (2005). Diversity and Functional Analysis of Bacterial Communities Associated with Natural Hydrocarbon Seeps in Acidic Soils at Rainbow Springs, Yellowstone National Park. Appl. Environ. Microbiol. 71: 5943-5950 [Abstract] [Full Text]  
• Tuffin, I. M., de Groot, P., Deane, S. M., Rawlings, D. E. (2005). An unusual Tn21-like transposon containing an ars operon is present in highly arsenic-resistant strains of the biomining bacterium Acidithiobacillus caldus. Microbiology 151: 3027-3039 [Abstract] [Full Text]  
• Papineau, D., Walker, J. J., Mojzsis, S. J., Pace, N. R. (2005). Composition and Structure of Microbial Communities from Stromatolites of Hamelin Pool in Shark Bay, Western Australia. Appl. Environ. Microbiol. 71: 4822-4832 [Abstract] [Full Text]  
• Bruscella, P., Cassagnaud, L., Ratouchniak, J., Brasseur, G., Lojou, E., Amils, R., Bonnefoy, V. (2005). The HiPIP from the acidophilic Acidithiobacillus ferrooxidans is correctly processed and translocated in Escherichia coli, in spite of the periplasm pH difference between these two micro-organisms. Microbiology 151: 1421-1431 [Abstract] [Full Text]  
• Chouari, R., Le Paslier, D., Dauga, C., Daegelen, P., Weissenbach, J., Sghir, A. (2005). Novel Major Bacterial Candidate Division within a Municipal Anaerobic Sludge Digester. Appl. Environ. Microbiol. 71: 2145-2153 [Abstract] [Full Text]  
• Backhed, F., Ley, R. E., Sonnenburg, J. L., Peterson, D. A., Gordon, J. I. (2005). Host-Bacterial Mutualism in the Human Intestine. Science 307: 1915-1920 [Abstract] [Full Text]  
• Broderick, N. A., Raffa, K. F., Goodman, R. M., Handelsman, J. (2004). Census of the Bacterial Community of the Gypsy Moth Larval Midgut by Using Culturing and Culture-Independent Methods. Appl. Environ. Microbiol. 70: 293-300 [Abstract] [Full Text]  
• Gonzalez-Toril, E., Llobet-Brossa, E., Casamayor, E. O., Amann, R., Amils, R. (2003). Microbial Ecology of an Extreme Acidic Environment, the Tinto River. Appl. Environ. Microbiol. 69: 4853-4865 [Abstract] [Full Text]  
• Dopson, M., Baker-Austin, C., Koppineedi, P. R., Bond, P. L. (2003). Growth in sulfidic mineral environments: metal resistance mechanisms in acidophilic micro-organisms. Microbiology 149: 1959-1970 [Abstract] [Full Text]  
• de la Torre, J. R., Goebel, B. M., Friedmann, E. I., Pace, N. R. (2003). Microbial Diversity of Cryptoendolithic Communities from the McMurdo Dry Valleys, Antarctica. Appl. Environ. Microbiol. 69: 3858-3867 [Abstract] [Full Text]  
• Okibe, N., Gericke, M., Hallberg, K. B., Johnson, D. B. (2003). Enumeration and Characterization of Acidophilic Microorganisms Isolated from a Pilot Plant Stirred-Tank Bioleaching Operation. Appl. Environ. Microbiol. 69: 1936-1943 [Abstract] [Full Text]  
• Frias-Lopez, J., Zerkle, A. L., Bonheyo, G. T., Fouke, B. W. (2002). Partitioning of Bacterial Communities between Seawater and Healthy, Black Band Diseased, and Dead Coral Surfaces. Appl. Environ. Microbiol. 68: 2214-2228 [Abstract] [Full Text]  
• Coram, N. J., Rawlings, D. E. (2002). Molecular Relationship between Two Groups of the Genus Leptospirillum and the Finding that Leptospirillum ferriphilum sp. nov. Dominates South African Commercial Biooxidation Tanks That Operate at 40{degrees}C. Appl. Environ. Microbiol. 68: 838-845 [Abstract] [Full Text]  
• Utaker, J. B., Andersen, K., Aakra, A., Moen, B., Nes, I. F. (2002). Phylogeny and Functional Expression of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase from the Autotrophic Ammonia-Oxidizing Bacterium Nitrosospira sp.Isolate 40KI. J. Bacteriol. 184: 468-478 [Abstract] [Full Text]  
• Bond, P. L., Druschel, G. K., Banfield, J. F. (2000). Comparison of Acid Mine Drainage Microbial Communities in Physically and Geochemically Distinct Ecosystems. Appl. Environ. Microbiol. 66: 4962-4971 [Abstract] [Full Text]  
• Bond, P. L., Smriga, S. P., Banfield, J. F. (2000). Phylogeny of Microorganisms Populating a Thick, Subaerial, Predominantly Lithotrophic Biofilm at an Extreme Acid Mine Drainage Site. Appl. Environ. Microbiol. 66: 3842-3849 [Abstract] [Full Text]  
• Peccia, J., Marchand, E. A., Silverstein, J., Hernandez, M. (2000). Development and Application of Small-Subunit rRNA Probes for Assessment of Selected Thiobacillus Species and Members of the Genus Acidiphilium. Appl. Environ. Microbiol. 66: 3065-3072 [Abstract] [Full Text]  
• Fife, D. J., Bruhn, D. F., Miller, K. S., Stoner, D. L. (2000). Evaluation of a Fluorescent Lectin-Based Staining Technique for Some Acidophilic Mining Bacteria. Appl. Environ. Microbiol. 66: 2208-2210 [Abstract] [Full Text]  
• Küsel, K., Dorsch, T., Acker, G., Stackebrandt, E. (1999). Microbial Reduction of Fe(III) in Acidic Sediments: Isolation of Acidiphilium cryptum JF-5 Capable of Coupling the Reduction of Fe(III) to the Oxidation of Glucose. Appl. Environ. Microbiol. 65: 3633-3640 [Abstract] [Full Text]