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Appl Environ Microbiol. 1994 June; 60(6): 1921-1926

In situ detection of hepatitis A virus in cell cultures and shellfish tissues.

Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.

ABSTRACT

An in situ transcription method was developed to detect hepatitis A virus RNA in both cell cultures and shellfish tissues. Radiolabeled cDNA copies were synthesized in situ by reverse transcriptase-directed transcription after annealing with a specific primer to the viral RNA. Both tritium (3H) and 35S were useful in the in situ transcription reaction, but the use of 3H resulted in a lower background and finer detail in the localization of viral particles. Application of the method to different organs of oysters which had bioaccumulated hepatitis A virus allowed the first in situ localization of the virus, specifically in stomach and hepatopancreatic tissues.

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