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Appl Environ Microbiol. 1994 July; 60(7): 2647-2649

Small, acid-soluble proteins bound to DNA protect Bacillus subtilis spores from being killed by freeze-drying.

Department of Applied Biochemistry and Food Science, University of Nottingham, Loughborough, Leicestershire, United Kingdom.

ABSTRACT

Wild-type spores of Bacillus subtilis were resistant to eight cycles of freeze-drying, whereas about 90% of spores lacking the two major DNA-binding proteins (small, acid-soluble proteins alpha and beta) were killed by three to four cycles of freeze-dryings, with significant mutagenesis and DNA damage accompanying the killing. This role for alpha/beta-type small, acid-soluble proteins in spore resistance to freeze-drying may be important in spore survival in the environment.

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