AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duetz, W A
Right arrow Articles by van Andel, J G
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duetz, W A
Right arrow Articles by van Andel, J G
Agricola
Right arrow Articles by Duetz, W A
Right arrow Articles by van Andel, J G

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1994 August; 60(8): 2858-2863

Competition in chemostat culture between Pseudomonas strains that use different pathways for the degradation of toluene.

W A Duetz, C de Jong, P A Williams and J G van Andel

Laboratory for Waste Materials and Emissions, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.

ABSTRACT

Pseudomonas putida mt-2, P. cepacia G4, P. mendocina KR1, and P. putida F1 degrade toluene through different pathways. In this study, we compared the competition behaviors of these strains in chemostat culture at a low growth rate (D = 0.05 h-1), with toluene as the sole source of carbon and energy. Either toluene or oxygen was growth limiting. Under toluene-limiting conditions, P. mendocina KR1, in which initial attack is by monooxygenation of the aromatic nucleus at the para position, outcompeted the other three strains. Under oxygen limitation, P. cepacia G4, which hydroxylates toluene in the ortho position, was the most competitive strain. P. putida mt-2, which metabolizes toluene via oxidation of the methyl group, was the least competitive strain under both growth conditions. The apparent superiority of strains carrying toluene degradation pathways that start degradation by hydroxylation of the aromatic nucleus was also found during competition experiments with pairs of strains of P. cepacia, P. fluorescence, and P. putida that were freshly isolated from contaminated soil.

Appl Environ Microbiol. 1994 August; 60(8): 2858-2863




This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1994 by the American Society for Microbiology. All rights reserved.