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Appl. Environ. Microbiol., Jan 1995, 44-47, Vol 61, No. 1
Copyright © 1995, American Society for Microbiology

Marine Vibrio Species Produce the Volatile Organic Compound Acetone

M Nemecek-Marshall, C Wojciechowski, J Kuzma, GM Silver and R Fall
Department of Chemistry and Biochemistry and Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado 80309-0215

While screening aerobic, heterotrophic marine bacteria for production of volatile organic compounds, we found that a group of isolates produced substantial amounts of acetone. Acetone production was confirmed by gas chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography. The major acetone producers were identified as nonclinical Vibrio species. Acetone production was maximal in the stationary phase of growth and was stimulated by addition of l-leucine but not the other common amino acids, suggesting that leucine degradation leads to acetone formation. Acetone production by marine vibrios may contribute to the dissolved organic carbon associated with phytoplankton, and some of the acetone produced may be volatilized to the atmosphere.


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