This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lloret, J. Articles by Rivilla, R. Search for Related Content PubMed PubMed Citation Articles by Lloret, J. Articles by Rivilla, R. Agricola Articles by Lloret, J. Articles by Rivilla, R.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Oct 1995, 3701-3704, Vol 61, No. 10
Copyright © 1995, American Society for Microbiology

Ionic Stress and Osmotic Pressure Induce Different Alterations in the Lipopolysaccharide of a Rhizobium meliloti Strain

J Lloret, L Bolanos, MM Lucas, JM Peart, NJ Brewin, I Bonilla and R Rivilla
Departamento de Biologia, Universidad Autonoma de Madrid, 28049 Madrid, and Departamento de Fisiologia y Bioquimica Vegetal, Centro de Ciencias Medioambientales, Consejo Superior de Investigaciones Cientificas, 28006 Madrid, Spain, and Department of Genetics and Monoclonal Antibody Department, John Innes Centre, Norwich Research Park, NR4 7UH, United Kingdom

A halotolerant strain of Rhizobium meliloti was isolated from nodules of a Melilotus plant growing in a salt marsh in Donana National Park (southwest Spain). This strain, EFB1, is able to grow at NaCl concentrations of up to 500 mM, and no effect on growth is produced by 300 mM NaCl. EFB1 showed alterations on its lipopolysaccharide (LPS) structure that can be related to salt stress: (i) silver-stained electrophoretic profiles showed a different mobility that was dependent on ionic stress but not on osmotic pressure, and (ii) a monoclonal antibody, JIM 40, recognized changes in LPS that were dependent on osmotic stress. Both modifications on LPS may form part of the adaptive mechanism of this bacterium for saline environments.

This article has been cited by other articles:

• Vriezen, J. A. C., de Bruijn, F. J., Nusslein, K. (2007). Responses of Rhizobia to Desiccation in Relation to Osmotic Stress, Oxygen, and Temperature. Appl. Environ. Microbiol. 73: 3451-3459 [Full Text]  
• Dominguez-Ferreras, A., Perez-Arnedo, R., Becker, A., Olivares, J., Soto, M. J., Sanjuan, J. (2006). Transcriptome Profiling Reveals the Importance of Plasmid pSymB for Osmoadaptation of Sinorhizobium meliloti. J. Bacteriol. 188: 7617-7625 [Abstract] [Full Text]  
• Miller-Williams, M., Loewen, P. C., Oresnik, I. J. (2006). Isolation of salt-sensitive mutants of Sinorhizobium meliloti strain Rm1021. Microbiology 152: 2049-2059 [Abstract] [Full Text]  
• Touze, T., Goude, R., Georgeault, S., Blanco, C., Bonnassie, S. (2004). Erwinia chrysanthemi O Antigen Is Required for Betaine Osmoprotection in High-Salt Media. J. Bacteriol. 186: 5547-5550 [Abstract] [Full Text]  
• Sanchez-Contreras, M., Martin, M., Villacieros, M., O'Gara, F., Bonilla, I., Rivilla, R. (2002). Phenotypic Selection and Phase Variation Occur during Alfalfa Root Colonization by Pseudomonas fluorescens F113. J. Bacteriol. 184: 1587-1596 [Abstract] [Full Text]  
• Sánchez-Contreras, M., Lloret, J., Martín, M., Villacieros, M., Bonilla, I., Rivilla, R. (2000). PCR Use of Highly Conserved DNA Regions for Identification of Sinorhizobium meliloti. Appl. Environ. Microbiol. 66: 3621-3623 [Abstract] [Full Text]  
• Pellock, B. J., Cheng, H.-P., Walker, G. C. (2000). Alfalfa Root Nodule Invasion Efficiency Is Dependent on Sinorhizobium meliloti Polysaccharides. J. Bacteriol. 182: 4310-4318 [Abstract] [Full Text]  
• Lloret, J., Wulff, B. B. H., Rubio, J. M., Downie, J. A., Bonilla, I., Rivilla, R. (1998). Exopolysaccharide II Production Is Regulated by Salt in the Halotolerant Strain Rhizobium meliloti EFB1. Appl. Environ. Microbiol. 64: 1024-1028 [Abstract] [Full Text]