This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liyanage, H. Articles by Bender, C. L. Search for Related Content PubMed PubMed Citation Articles by Liyanage, H. Articles by Bender, C. L. Agricola Articles by Liyanage, H. Articles by Bender, C. L.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Nov 1995, 3843-3848, Vol 61, No. 11
Copyright © 1995, American Society for Microbiology

Characterization and transcriptional analysis of the gene cluster for coronafacic acid, the polyketide component of the phytotoxin coronatine

H Liyanage, DA Palmer, M Ullrich and CL Bender
Department of Plant Pathology, Noble Research Center, Oklahoma State University, Stillwater 74078-3032, USA.

Coronafacic acid (CFA), the polyketide component of the phytotoxin coronatine (COR), is activated and coupled to coronamic acid via amide bond formation, a biosynthetic step presumably catalyzed by the CFA ligase (cfl) gene product. The COR biosynthetic gene cluster in Pseudomonas syringae pv. glycinea PG4180 is located within a 32-kb region of a 90-kb plasmid designated p4180A. In the present study, a cloned region of p4180A complemented all CFA- mutants spanning an 18.8- kb region of the COR biosynthetic cluster. The genetic evidence presented in this study indicates that cfl and the CFA biosynthetic gene cluster are encoded by a single transcript and that transcription of all of the genes in this operon is directed by the cfl promoter. The cfl promoter was localized to a 0.37-kb region upstream of the transcriptional start site by progressive subcloning in pRG960sd, a vector containing a promoterless glucuronidase gene. Transcription of the cfl/CFA operon was temperature sensitive and showed maximal glucuronidase activity at 18 degrees C. Furthermore, transcription of the cfl/CFA operon was dependent on the functional activity of a modified two-component regulatory system located within the COR biosynthetic gene cluster. Thermoregulation of the cfl/CFA operon and the coronamic acid biosynthetic gene cluster via the modified two- component regulatory system is discussed.

This article has been cited by other articles:

• Braun, Y., Smirnova, A. V., Schenk, A., Weingart, H., Burau, C., Muskhelishvili, G., Ullrich, M. S. (2008). Component and protein domain exchange analysis of a thermoresponsive, two-component regulatory system of Pseudomonas syringae. Microbiology 154: 2700-2708 [Abstract] [Full Text]  
• Seidle, H., Rangaswamy, V., Couch, R., Bender, C. L., Parry, R. J. (2004). Characterization of Cfa1, a Monofunctional Acyl Carrier Protein Involved in the Biosynthesis of the Phytotoxin Coronatine. J. Bacteriol. 186: 2499-2503 [Abstract] [Full Text]  
• Ullrich, M. S., Schergaut, M., Boch, J., Ullrich, B. (2000). Temperature-responsive genetic loci in the plant pathogen Pseudomonas syringae pv. glycinea. Microbiology 146: 2457-2468 [Abstract] [Full Text]  
• Bender, C. L., Alarcon-Chaidez, F., Gross, D. C. (1999). Pseudomonas syringae Phytotoxins: Mode of Action, Regulation, and Biosynthesis by Peptide and Polyketide Synthetases. Microbiol. Mol. Biol. Rev. 63: 266-292 [Abstract] [Full Text]  
• Bangera, M. G., Thomashow, L. S. (1999). Identification and Characterization of a Gene Cluster for Synthesis of the Polyketide Antibiotic 2,4-Diacetylphloroglucinol from Pseudomonas fluorescens Q2-87. J. Bacteriol. 181: 3155-3163 [Abstract] [Full Text]  
• Rangaswamy, V., Jiralerspong, S., Parry, R., Bender, C. L. (1998). Biosynthesis of the Pseudomonas polyketide coronafacic acid requires monofunctional and multifunctional polyketide synthase proteins. Proc. Natl. Acad. Sci. USA 95: 15469-15474 [Abstract] [Full Text]  
• Peñaloza-Vázquez, A., Bender, C. L. (1998). Characterization of CorR, a Transcriptional Activator Which Is Required for Biosynthesis of the Phytotoxin Coronatine. J. Bacteriol. 180: 6252-6259 [Abstract] [Full Text]  
• Rangaswamy, V., Mitchell, R., Ullrich, M., Bender, C. (1998). Analysis of Genes Involved in Biosynthesis of Coronafacic Acid, the Polyketide Component of the Phytotoxin Coronatine. J. Bacteriol. 180: 3330-3338 [Abstract] [Full Text]  
• Budde, I. P., Rohde, B. H., Bender, C. L., Ullrich, M. S. (1998). Growth Phase and Temperature Influence Promoter Activity, Transcript Abundance, and Protein Stability during Biosynthesis of the Pseudomonas syringae Phytotoxin Coronatine. J. Bacteriol. 180: 1360-1367 [Abstract] [Full Text]