This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Herman, L. M.
Right arrow Articles by Waes, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herman, L. M.
Right arrow Articles by Waes, G. M.
Agricola
Right arrow Articles by Herman, L. M.
Right arrow Articles by Waes, G. M.

Next Article 

Appl. Environ. Microbiol., Dec 1995, 4141-4146, Vol 61, No. 12
Copyright © 1995, American Society for Microbiology

A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk

LM Herman, JH De Block and GM Waes
Government Dairy Research Station, Agricultural Research Centre Ghent, Melle, Belgium.

A direct detection method for Clostridium tyrobutyricum spores in up to 100 ml of raw milk is presented. The bacterial spores are concentrated by centrifugation after chemical extraction of the milk components. The vegetative cells are selectively lysed, and their DNA is digested and washed away. Afterwards, the DNA is liberated from the spores by microwave treatment. For the identification of the C. tyrobutyricum DNA, a two-step PCR method with two nested pairs of primers is used. The primers were derived from the 16S-23S rRNA spacer region of C. tyrobutyricum, and the specificity of each of them for C. tyrobutyricum is demonstrated. The detection limit can be estimated to be between 3 and 30 spores in 100 ml of raw milk.


This article has been cited by other articles:

  • Julien, M.-C., Dion, P., Lafreniere, C., Antoun, H., Drouin, P. (2008). Sources of Clostridia in Raw Milk on Farms. Appl. Environ. Microbiol. 74: 6348-6357 [Abstract] [Full Text]  
  • Lopez-Enriquez, L., Rodriguez-Lazaro, D., Hernandez, M. (2007). Quantitative Detection of Clostridium tyrobutyricum in Milk by Real-Time PCR. Appl. Environ. Microbiol. 73: 3747-3751 [Abstract] [Full Text]  
  • Warsen, A. E., Krug, M. J., LaFrentz, S., Stanek, D. R., Loge, F. J., Call, D. R. (2004). Simultaneous Discrimination between 15 Fish Pathogens by Using 16S Ribosomal DNA PCR and DNA Microarrays. Appl. Environ. Microbiol. 70: 4216-4221 [Abstract] [Full Text]  
  • Lucore, L. A., Cullison, M. A., Jaykus, L.-A. (2000). Immobilization with Metal Hydroxides as a Means To Concentrate Food-Borne Bacteria for Detection by Cultural and Molecular Methods. Appl. Environ. Microbiol. 66: 1769-1776 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»