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Appl. Environ. Microbiol., Mar 1995, 1138-1140, Vol 61, No. 3
Copyright © 1995, American Society for Microbiology

Minimal Amino Acid Requirements of the Hyperthermophilic Archaeon Pyrococcus abyssi, Isolated from Deep-Sea Hydrothermal Vents

L Watrin, V Martin-Jezequel and D Prieur
Department of Microbiology, Station Biologique, Centre National de la Recherche Scientifique Unite Propre de Recherche 4601, F-29680 Roscoff, France

A minimal growth medium containing only nine amino acids and vitamins as the sole carbon and energy sources allowed the growth of Pyrococcus abyssi GE 5, a novel hyperthermophilic sulfur-metabolizing archaeon isolated from deep-sea hydrothermal vents. The generation time in this medium was about 40 min, and cell densities up to 5 x 10(sup8) cells ml(sup-1) were attained. These results are similar to those obtained previously with complex proteinaceous media.


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