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Appl. Environ. Microbiol., Apr 1995, 1187-1193, Vol 61, No. 4
Copyright © 1995, American Society for Microbiology

Wide Distribution of Mitochondrial Genome Rearrangements in Wild Strains of the Cultivated Basidiomycete Agrocybe aegerita

G Barroso, S Blesa and J Labarere
Laboratoire de Genetique Moleculaire et dAmelioration des Champignons Cultives, Universite de Bordeaux II Institut National de la Recherche Agronomique, Centre de Recherche Agronomique de Bordeaux, 33883 Villenave dOrnon Cedex, France

We used restriction fragment length polymorphisms to examine mitochondrial genome rearrangements in 36 wild strains of the cultivated basidiomycete Agrocybe aegerita, collected from widely distributed locations in Europe. We identified two polymorphic regions within the mitochondrial DNA which varied independently: one carrying the Cox II coding sequence and the other carrying the Cox I, ATP6, and ATP8 coding sequences. Two types of mutations were responsible for the restriction fragment length polymorphisms that we observed and, accordingly, were involved in the A. aegerita mitochondrial genome evolution: (i) point mutations, which resulted in strain-specific mitochondrial markers, and (ii) length mutations due to genome rearrangements, such as deletions, insertions, or duplications. Within each polymorphic region, the length differences defined only two mitochondrial types, suggesting that these length mutations were not randomly generated but resulted from a precise rearrangement mechanism. For each of the two polymorphic regions, the two molecular types were distributed among the 36 strains without obvious correlation with their geographic origin. On the basis of these two polymorphisms, it is possible to define four mitochondrial haplotypes. The four mitochondrial haplotypes could be the result of intermolecular recombination between allelic forms present in the population long enough to reach linkage equilibrium. All of the 36 dikaryotic strains contained only a single mitochondrial type, confirming the previously described mitochondrial sorting out after cytoplasmic mixing in basidiomycetes.

This article has been cited by other articles:

• Barroso, G., Bois, F., Labarère, J. (2001). Duplication of a Truncated Paralog of the Family B DNA Polymerase Gene Aa-polB in the Agrocybe aegerita Mitochondrial Genome. Appl. Environ. Microbiol. 67: 1739-1743 [Abstract] [Full Text]  
• Saville, B. J., Kohli, Y., Anderson, J. B. (1998). mtDNA recombination in a natural population. Proc. Natl. Acad. Sci. USA 95: 1331-1335 [Abstract] [Full Text]