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Appl. Environ. Microbiol., 04 1995, 1318-1322, Vol 61, No. 4
Copyright © 1995, American Society for Microbiology

Degradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by Stenotrophomonas maltophilia PB1

PR Binks, S Nicklin and NC Bruce
Institute of Biotechnology, University of Cambridge, United Kingdom.

A mixed microbial culture capable of metabolizing the explosive RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) was obtained from soil enrichments under aerobic and nitrogen-limiting conditions. A bacterium, Stenotrophomonas maltophilia PB1, isolated from the culture used RDX as a sole source of nitrogen for growth. Three moles of nitrogen was used per mole of RDX, yielding a metabolite identified by mass spectroscopy and 1H nuclear magnetic resonance analysis as methylene-N-(hydroxymethyl)-hydroxylamine-N'-(hydroxymethyl)nitroamin e. The bacterium also used s-triazine as a sole source of nitrogen but not the structurally similar compounds octahydro-1,3,5,7-tetranitro- 1,3,5,7-tetrazocine, cyanuric acid, and melamine. An inducible RDX- degrading activity was present in crude cell extracts.

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