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Appl. Environ. Microbiol., Apr 1995, 1658-1660, Vol 61, No. 4
NA Miner, GK Mulberry, AN Starks, A Powers-Prather, M Entrup, M Armstrong and B Maida
Two laboratories tested four different brands of alkaline 2% glutaraldehyde
sterilants by the Association of Official Analytical Chemists sporicidal
test. Each laboratory found survival of Clostridium sporogenes spores on
spore-labeled unglazed porcelain penicylinders (cylinders) to vary from
test to test, and survival did not always correlate with increasing
sterilant exposure time. These results were consistent with a theory that
there may be random conditions within the test that prevent the sterilant
from contacting all spores. Further studies indicated that the prior
history of the unglazed porcelain cylinders and whether the C. sporogenes
culture grown in egg-meat media had been processed (homogenized) to
eliminate visible pieces of egg-meat media were important factors affecting
the results and repeatability of this test.
Copyright © 1995, American Society for Microbiology
Identification of Possible Artifacts in the Association of Official Analytical Chemists Sporicidal Test
MicroChem Laboratory, Fort Worth, Texas 76118, and Hill Top Biolabs, Inc., Cincinnati, Ohio 45242
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