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Appl. Environ. Microbiol., May 1995, 1968-1975, Vol 61, No. 5
Copyright © 1995, American Society for Microbiology

Melanin Production by a Filamentous Soil Fungus in Response to Copper and Localization of Copper Sulfide by Sulfide-Silver Staining

T Caesar-Tonthat, F Van Ommen Kloeke, GG Geesey and JM Henson
Department of Microbiology and Engineering Research Center, Montana State University, Bozeman, Montana 59717

Gaeumannomyces graminis var. graminis, a filamentous soil ascomycete, exhibited enhanced cell wall melanin accumulation when exposed to as little as 0.01 mM CuSO(inf4) in minimal broth culture. Because its synthesis was inhibited by tricyclazole, the melanin produced in response to copper was dihydroxynaphthalene melanin. An additional hyphal cell wall layer was visualized by electron microscopy when hyphae were grown in the presence of copper and fixed by cryotechniques. This electron-dense layer was between the outer cell wall and the inner chitin layer and doubled the total wall thickness. In copper-grown cells that were also treated with tricyclazole, this electron-dense layer was absent. Atomic absorption spectroscopy demonstrated that up to 3.5 mg of Cu per g of fungal mycelium was adsorbed or taken up by hyphae grown in 0.06 mM CuSO(inf4). A method for silver enhancement was developed to determine the cellular location of CuS. CuS was present in cell walls and septa of copper-grown hyphae. Electron microscopy of silver-stained cells suggested that CuS was associated with the melanin layer of cell walls.


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