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Appl. Environ. Microbiol., May 1995, 2016-2019, Vol 61, No. 5
Copyright © 1995, American Society for Microbiology

A New Solid Medium for Enumerating Cellulose-Utilizing Bacteria in Soil

CW Hendricks, JD Doyle and B Hugley
Environmental Research Laboratory, U.S. Environmental Protection Agency, and ManTech Environmental Technology, Inc., Corvallis, Oregon 97333

A solid medium containing ashed, acid-washed cellulose and a dye, Congo red, has been developed for enumeration of cellulose-utilizing bacteria in soil. Bacteria able to use the cellulose in this medium produced distinct zones of clearing around their colonies. A vivid contrast between the uniform red color of the medium and these halos made this method of differentiation of these organisms superior to other methods.

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