This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Toba, T.
Right arrow Articles by Korhonen, T. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Toba, T.
Right arrow Articles by Korhonen, T. K.
Agricola
Right arrow Articles by Toba, T.
Right arrow Articles by Korhonen, T. K.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Jul 1995, 2467-2471, Vol 61, No. 7
Copyright © 1995, American Society for Microbiology

A Collagen-Binding S-Layer Protein in Lactobacillus crispatus

T Toba, R Virkola, B Westerlund, Y Bjorkman, J Sillanpaa, T Vartio, N Kalkkinen and TK Korhonen
Division of General Microbiology, Department of Biosciences, Department of Pathology, and Institute of Biotechnology, University of Helsinki, SF-00014 Helsinki, Finland, and Department of Science of Bioresources, Faculty of Agriculture, Hirosaki University, 036 Hirosaki, Japan

Two S-layer-expressing strains, Lactobacillus crispatus JCM 5810 and Lactobacillus acidophilus JCM 1132, were assessed for adherence to proteins of the mammalian extracellular matrix. L. crispatus JCM 5810 adhered efficiently to immobilized type IV and I collagens, laminin, and, with a lower affinity, to type V collagen and fibronectin. Strain JCM 1132 did not exhibit detectable adhesiveness. Within the fibronectin molecule, JCM 5810 recognized the 120-kDa cell-binding fragment of the protein, while no bacterial adhesion to the amino-terminal 30-kDa or the gelatin-binding 40-kDa fragment was detected. JCM 5810 but not JCM 1132 also bound (sup125)I-labelled soluble type IV collagen, and this binding was efficiently inhibited by unlabelled type IV and I collagens and less efficiently by type V collagen, but not by laminin or fibronectin. L. crispatus JCM 5810 but not L. acidophilus JCM 1132 also adhered to Matrigel, a reconstituted basement membrane preparation from mouse sarcoma cells, as well as to the extracellular matrix prepared from human Intestine 407 cells. S-layers from both strains were extracted with 2 M guanidine hydrochloride, separated by electrophoresis, and transferred to nitrocellulose sheets. The S-layer protein from JCM 5810 bound (sup125)I-labelled type IV collagen, whereas no binding was seen with the S-layer protein from JCM 1132. Binding of (sup125)I-collagen IV to the JCM 5810 S-layer protein was effectively inhibited by unlabelled type I and IV collagens but not by type V collagen, laminin, or fibronectin. It was concluded that L. crispatus JCM 5810 has the capacity to adhere to human subintestinal extracellular matrix via a collagen-binding S-layer.


This article has been cited by other articles:

  • Schar-Zammaretti, P., Dillmann, M.-L., D'Amico, N., Affolter, M., Ubbink, J. (2005). Influence of Fermentation Medium Composition on Physicochemical Surface Properties of Lactobacillus acidophilus. Appl. Environ. Microbiol. 71: 8165-8173 [Abstract] [Full Text]  
  • Kumura, H., Tanoue, Y., Tsukahara, M., Tanaka, T., Shimazaki, K. (2004). Screening of Dairy Yeast Strains for Probiotic Applications. J DAIRY SCI 87: 4050-4056 [Abstract] [Full Text]  
  • Avall-Jaaskelainen, S., Lindholm, A., Palva, A. (2003). Surface Display of the Receptor-Binding Region of the Lactobacillusbrevis S-Layer Protein in Lactococcuslactis Provides Nonadhesive Lactococci with the Ability To Adhere to Intestinal Epithelial Cells. Appl. Environ. Microbiol. 69: 2230-2236 [Abstract] [Full Text]  
  • Jakava-Viljanen, M., Avall-Jaaskelainen, S., Messner, P., Sleytr, U. B., Palva, A. (2002). Isolation of Three New Surface Layer Protein Genes (slp) from Lactobacillus brevis ATCC 14869 and Characterization of the Change in Their Expression under Aerated and Anaerobic Conditions. J. Bacteriol. 184: 6786-6795 [Abstract] [Full Text]  
  • Avall-Jaaskelainen, S., Kyla-Nikkila, K., Kahala, M., Miikkulainen-Lahti, T., Palva, A. (2002). Surface Display of Foreign Epitopes on the Lactobacillus brevis S-Layer. Appl. Environ. Microbiol. 68: 5943-5951 [Abstract] [Full Text]  
  • Ventura, M., Jankovic, I., Walker, D. C., Pridmore, R. D., Zink, R. (2002). Identification and Characterization of Novel Surface Proteins in Lactobacillus johnsonii and Lactobacillus gasseri. Appl. Environ. Microbiol. 68: 6172-6181 [Abstract] [Full Text]  
  • Edelman, S., Westerlund-Wikstrom, B., Leskela, S., Kettunen, H., Rautonen, N., Apajalahti, J., Korhonen, T. K. (2002). In Vitro Adhesion Specificity of Indigenous Lactobacilli within the Avian Intestinal Tract. Appl. Environ. Microbiol. 68: 5155-5159 [Abstract] [Full Text]  
  • Calabi, E., Calabi, F., Phillips, A. D., Fairweather, N. F. (2002). Binding of Clostridium difficile Surface Layer Proteins to Gastrointestinal Tissues. Infect. Immun. 70: 5770-5778 [Abstract] [Full Text]  
  • Hynonen, U., Westerlund-Wikstrom, B., Palva, A., Korhonen, T. K. (2002). Identification by Flagellum Display of an Epithelial Cell- and Fibronectin-Binding Function in the SlpA Surface Protein of Lactobacillus brevis. J. Bacteriol. 184: 3360-3367 [Abstract] [Full Text]  
  • Rojas, M., Ascencio, F., Conway, P. L. (2002). Purification and Characterization of a Surface Protein from Lactobacillus fermentum 104R That Binds to Porcine Small Intestinal Mucus and Gastric Mucin. Appl. Environ. Microbiol. 68: 2330-2336 [Abstract] [Full Text]  
  • ANNUK, H., HYNES, S. O., HIRMO, S., MIKELSAAR, M., WADSTROM, T. (2001). Characterisation and differentiation of lactobacilli by lectin typing. J Med Microbiol 50: 1069-1074 [Abstract] [Full Text]  
  • Martínez, B., Sillanpää, J., Smit, E., Korhonen, T. K., Pouwels, P. H. (2000). Expression of cbsA Encoding the Collagen-Binding S-Protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T. J. Bacteriol. 182: 6857-6861 [Abstract] [Full Text]  
  • Sillanpää, J., Martínez, B., Antikainen, J., Toba, T., Kalkkinen, N., Tankka, S., Lounatmaa, K., Keränen, J., Höök, M., Westerlund-Wikström, B., Pouwels, P. H., Korhonen, T. K. (2000). Characterization of the Collagen-Binding S-Layer Protein CbsA of Lactobacillus crispatus. J. Bacteriol. 182: 6440-6450 [Abstract] [Full Text]  
  • Neeser, J.-R., Granato, D., Rouvet, M., Servin, A., Teneberg, S., Karlsson, K.-A. (2000). Lactobacillus johnsonii La1 shares carbohydrate-binding specificities with several enteropathogenic bacteria. Glycobiology 10: 1193-1199 [Abstract] [Full Text]  
  • Granato, D., Perotti, F., Masserey, I., Rouvet, M., Golliard, M., Servin, A., Brassart, D. (1999). Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsonii La1 to Human Enterocyte-Like Caco-2 Cells. Appl. Environ. Microbiol. 65: 1071-1077 [Abstract] [Full Text]  
  • Kotiranta, A., Haapasalo, M., Kari, K., Kerosuo, E., Olsen, I., Sorsa, T., Meurman, J. H., Lounatmaa, K. (1998). Surface Structure, Hydrophobicity, Phagocytosis, and Adherence to Matrix Proteins of Bacillus cereus Cells with and without the Crystalline Surface Protein Layer. Infect. Immun. 66: 4895-4902 [Abstract] [Full Text]  
  • Eberhard, T., Virkola, R., Korhonen, T., Kronvall, G., Ullberg, M. (1998). Binding to Human Extracellular Matrix by Neisseria meningitidis. Infect. Immun. 66: 1791-1794 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»