This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Morgan, S. Articles by Hill, C. Search for Related Content PubMed PubMed Citation Articles by Morgan, S. Articles by Hill, C. Agricola Articles by Morgan, S. Articles by Hill, C.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Aug 1995, 2995-3001, Vol 61, No. 8
Copyright © 1995, American Society for Microbiology

Bacteriolytic activity caused by the presence of a novel lactococcal plasmid encoding lactococcins A, B, and M

S Morgan, RP Ross and C Hill
National Dairy Products Research Centre, Fermoy, Republic of Ireland.

Lactococcus lactis subsp. lactis biovar diacetylactis DPC938 was identified as a bacteriocin-producing strain which exhibited a bacteriolytic effect on other lactococci. Lysis of such target strains was associated with decreases in optical density and release of the intracellular enzyme lactate dehydrogenase. DPC938 exhibits cross- immunity to L. lactis subsp. cremoris 9B4 (M.J. van Belkum, B.J. Hayema, A. Geis, J. Kok, and G. Venema, Appl. Environ. Microbiol. 55:1187-1191, 1989), a strain which produces the bacteriocins lactococcins A, B, and M. Genetic analyses revealed that a 15.5-kb region of DNA encoding these bacteriocins is highly conserved in 9B4, DPC938, and DPC3286, an overproducing derivative of DPC938. This region is located on a 72- and a 78-kb nonmobilizable plasmid in DPC938 and DPC3286, respectively. The bacteriolytic effect exhibited by DPC938 and DPC3286 on sensitive cultures is most probably due to the concerted action of all three bacteriocins. Since these cultures exhibit a lytic effect on lactococci, they have a potential application in the dairy industry as accelerators of starter lysis and hence accelerators of cheese ripening.

This article has been cited by other articles:

• Fontaine, L., Hols, P. (2008). The Inhibitory Spectrum of Thermophilin 9 from Streptococcus thermophilus LMD-9 Depends on the Production of Multiple Peptides and the Activity of BlpGSt, a Thiol-Disulfide Oxidase. Appl. Environ. Microbiol. 74: 1102-1110 [Abstract] [Full Text]  
• Wirawan, R. E., Swanson, K. M., Kleffmann, T., Jack, R. W., Tagg, J. R. (2007). Uberolysin: a novel cyclic bacteriocin produced by Streptococcus uberis. Microbiology 153: 1619-1630 [Abstract] [Full Text]  
• Zendo, T., Koga, S., Shigeri, Y., Nakayama, J., Sonomoto, K. (2006). Lactococcin Q, a Novel Two-Peptide Bacteriocin Produced by Lactococcus lactis QU 4.. Appl. Environ. Microbiol. 72: 3383-3389 [Abstract] [Full Text]  
• Garde, S., Avila, M., Gaya, P., Medina, M., Nunez, M. (2006). Proteolysis of Hispanico cheese manufactured using lacticin 481-producing Lactococcus lactis ssp. lactis INIA 639.. J DAIRY SCI 89: 840-849 [Abstract] [Full Text]  
• O'Sullivan, L., Morgan, S. M., Ross, R. P., Hill, C. (2002). Elevated Enzyme Release from Lactococcal Starter Cultures on Exposure to the Lantibiotic Lacticin 481, Produced by Lactococcus lactis DPC5552. J DAIRY SCI 85: 2130-2140 [Abstract] [Full Text]  
• Gänzle, M. G., Höltzel, A., Walter, J., Jung, G., Hammes, W. P. (2000). Characterization of Reutericyclin Produced by Lactobacillus reuteri LTH2584. Appl. Environ. Microbiol. 66: 4325-4333 [Abstract] [Full Text]  
• Martínez-Cuesta, M. C., Kok, J., Herranz, E., Peláez, C., Requena, T., Buist, G. (2000). Requirement of Autolytic Activity for Bacteriocin-Induced Lysis. Appl. Environ. Microbiol. 66: 3174-3179 [Abstract] [Full Text]  
• O'Sullivan, D., Ross, R. P., Fitzgerald, G. F., Coffey, A. (2000). Investigation of the Relationship between Lysogeny and Lysis of Lactococcus lactis in Cheese Using Prophage-Targeted PCR. Appl. Environ. Microbiol. 66: 2192-2198 [Abstract] [Full Text]  
• O'Keeffe, T., Hill, C., Ross, R. P. (1999). Characterization and Heterologous Expression of the Genes Encoding Enterocin A Production, Immunity, and Regulation in Enterococcus faecium DPC1146. Appl. Environ. Microbiol. 65: 1506-1515 [Abstract] [Full Text]