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Appl. Environ. Microbiol., Aug 1995, 3008-3013, Vol 61, No. 8
Copyright © 1995, American Society for Microbiology

Genetic Structure of Acetobacter diazotrophicus Populations and Identification of a New Genetically Distant Group

J Caballero-Mellado, LE Fuentes-Ramirez, VM Reis and E Martinez-Romero
Departamento de Genetica Molecular, Centro de Investigacion sobre Fijacion de Nitrogeno, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico, and EMBRAPA, Km 47, Centro Nacional de Pesquisa de Agrobiologia, Seropedica 23851-970, Rio de Janeiro, Brazil

A total of 55 isolates of Acetobacter diazotrophicus recovered from diverse sucrose-rich host plants and from mealybugs associated with sugarcane plants were characterized by the electrophoretic mobilities of 12 metabolic enzymes. We identified seven different electrophoretic types (ETs), six of which are closely related within a genetic distance of 0.195 and exhibit high DNA-DNA homology. The seventh ET was largely divergent, separated at a genetic distance of 0.53, and had only 54% DNA homology to the reference strain. Strains corresponding to ET 7 could represent a distinct nitrogen-fixing species of the genus Acetobacter. More genetic diversity was found in isolates from Brazil than in those from Mexico, probably due to the very different crop nitrogen fertilization levels used.

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