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Appl. Environ. Microbiol., Sep 1995, 3460-3462, Vol 61, No. 9
Copyright © 1995, American Society for Microbiology

Genotyping of rotaviruses isolated from sewage

R Gajardo, N Bouchriti, RM Pinto and A Bosch
Department of Microbiology, University of Barcelona, Spain.

Rotaviruses from environmental samples have been genotyped by a seminested reverse transcription PCR assay with serotype-specific primers derived from variable regions of gene 9, which produce different characteristic segment sizes for serotypes 1 to 4. The method enabled the detection and identification of type 1, 2, and 3 group A rotaviruses in sewage.


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