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Appl. Environ. Microbiol., Sep 1995, 3494-3498, Vol 61, No. 9
Copyright © 1995, American Society for Microbiology

Survival and Activity of lux-Marked Aeromonas salmonicida in Seawater

Y Ferguson, LA Glover, DM McGillivray and JI Prosser
Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, Aberdeen AB9 1AS, and Scottish Office Agriculture and Fisheries Department Marine Laboratory, Aberdeen AB9 8DB, Scotland, United Kingdom

The fish pathogen Aeromonas salmonicida was chromosomally marked with genes encoding bacterial luciferase, luxAB, isolated from Vibrio fischeri, resulting in constitutive luciferase production. During exponential growth in liquid batch culture, luminescence was directly proportional to biomass concentration, and luminometry provided a lower detection limit of approximately 10(sup3) cells ml(sup-1), 1 order of magnitude more sensitive than enzyme-linked immunosorbent assay detection. In sterile seawater at 4(deg)C, lux-marked A. salmonicida entered a dormant, nonculturable state and population activity decreased rapidly. The activity per viable cell, however, increased by day 4, indicating that a proportion of the population remained active and culturable. Putative dormant cells were not resuscitated after the addition of a range of substrates.


This article has been cited by other articles:

  • Unge, A., Tombolini, R., Mølbak, L., Jansson, J. K. (1999). Simultaneous Monitoring of Cell Number and Metabolic Activity of Specific Bacterial Populations with a Dual gfp-luxAB Marker System. Appl. Environ. Microbiol. 65: 813-821 [Abstract] [Full Text]  


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