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Appl. Environ. Microbiol., Oct 1996, 3697-3703, Vol 62, No. 10
Copyright © 1996, American Society for Microbiology

Expression of lip genes during growth in soil and oxidation of anthracene by Phanerochaete chrysosporium

BW Bogan, B Schoenike, RT Lamar and D Cullen
Department of Cell and Molecular Biology, University of Wisconsin- Madison 53706, USA.

mRNA extraction from soil and quantitation by competitive reverse transcription-PCR were combined to study the expression of the 10 known lignin peroxidase (lip) genes in anthracene-transforming soil cultures of Phanerochaete chrysosporium. Levels of extractable lipA transcript and protein (LiP H8) were well correlated, although they were separated by a 2-day lag period. The patterns of transcript abundance over time in soil-grown P. chrysosporium varied among the nine lip mRNAs detected; comparison with lip gene expression under different liquid culture conditions suggested an early phase of carbon limitation for the cultures as a whole, which was followed by a transition to nitrogen starvation. Anthracene transformation occurred throughout the 25-day course of the experiment and, therefore, likely involves mechanisms distinct from those involved in oxidation of non-LiP substrate polycyclic aromatic hydrocarbons.

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