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Appl. Environ. Microbiol., Nov 1996, 4136-4146, Vol 62, No. 11
Copyright © 1996, American Society for Microbiology

Characterization and Differentiation of Filamentous Fungi Based on Fatty Acid Composition

PD Stahl and MJ Klug
Center for Microbial Ecology and Kellogg Biological Station, Michigan State University, Hickory Corners, Michigan 49060

Cellular fatty acid composition of 100 different filamentous fungi, including oomycetes, zygomycetes, ascomycetes, basidiomycetes, and sterile mycelia, was analyzed to determine if they can be differentiated from one another on this basis and how minor variations in culture temperature and age affect this characteristic. Many fungi were found to possess the same fatty acids but produced different relative concentrations of each. Some fungi differed in both the fatty acids produced and in the relative concentrations of others. Multivariate discriminant analysis demonstrated that all of the species included in this study had significantly different (P < 0.001) fatty acid profiles. Each of the three phyla from which representative species were analyzed and the sterile forms had distinctive fatty acid profiles. Significant differences in fatty acid composition were also found at the intraspecific level. Both culture temperature and age affected fatty acid composition in the fungi examined, but when these factors were held constant, variance in fatty acid composition was not a problem and fungal fatty acid profiles could be differentiated statistically.

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