This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tang, J. D. Articles by Peferoen, M. Search for Related Content PubMed PubMed Citation Articles by Tang, J. D. Articles by Peferoen, M. Agricola Articles by Tang, J. D. Articles by Peferoen, M.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Feb 1996, 564-569, Vol 62, No. 2
Copyright © 1996, American Society for Microbiology

Toxicity of Bacillus thuringiensis Spore and Crystal Protein to Resistant Diamondback Moth (Plutella xylostella)

JD Tang, AM Shelton, J Van Rie, S De Roeck, WJ Moar, RT Roush and M Peferoen
Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, New York 14456; Plant Genetic Systems, B-9000 Ghent, Belgium; Department of Entomology, Auburn University, Auburn, Alabama 36849; and Department of Entomology, Cornell University, Ithaca, New York 14853

A colony of Plutella xylostella from crucifer fields in Florida was used in mortality bioassays with HD-1 spore, CryIA(a), CryIA(b), CryIA(c), CryIB, CryIC, CryID, CryIE, or CryIIA. The data revealed high levels of field-evolved resistance to HD-1 spore and all CryIA protoxins and no resistance to CryIB, CryIC, or CryID. CryIE and CryIIA were essentially not toxic. When HD-1 spore was combined 1:1 with protoxin and fed to susceptible larvae, spore synergized the activity of CryIA and CryIC 5- to 8-fold and 1.7-fold, respectively, and did not synergize the mortality of CryIIA. When fed to Florida larvae, spore failed to synergize the activity of all three CryIA protoxins, synergized the activity of CryIC 5.3-fold, and did not synergize the mortality for CryIIA. Binding studies with CryIA(b), CryIB, and CryIC were performed to determine possible mechanisms of resistance. The two techniques used were (i) binding of biotinylated toxin to tissue sections of larval midguts and (ii) binding of biotinylated toxin to brush border membrane vesicles prepared from whole larvae. Both showed dramatically reduced binding of CryIA(b) in resistant larvae compared with that in susceptible larvae but no differences in binding of CryIB or CryIC.

This article has been cited by other articles:

• Tabashnik, B. E., Dennehy, T. J., Sims, M. A., Larkin, K., Head, G. P., Moar, W. J., Carriere, Y. (2002). Control of Resistant Pink Bollworm (Pectinophora gossypiella) by Transgenic Cotton That Produces Bacillus thuringiensis Toxin Cry2Ab. Appl. Environ. Microbiol. 68: 3790-3794 [Abstract] [Full Text]  
• Gonzalez-Cabrera, J., Herrero, S., Sayyed, A. H., Escriche, B., Liu, Y. B., Meyer, S. K., Wright, D. J., Tabashnik, B. E., Ferre, J. (2001). Variation in Susceptibility to Bacillus thuringiensis Toxins among Unselected Strains of Plutella xylostella. Appl. Environ. Microbiol. 67: 4610-4613 [Abstract] [Full Text]  
• Liu, Y.-B., Tabashnik, B. E., Meyer, S. K., Crickmore, N. (2001). Cross-Resistance and Stability of Resistance to Bacillus thuringiensis Toxin Cry1C in Diamondback Moth. Appl. Environ. Microbiol. 67: 3216-3219 [Abstract] [Full Text]  
• Zhao, J.-Z., Collins, H. L., Tang, J. D., Cao, J., Earle, E. D., Roush, R. T., Herrero, S., Escriche, B., Ferré, J., Shelton, A. M. (2000). Development and Characterization of Diamondback Moth Resistance to Transgenic Broccoli Expressing High Levels of Cry1C. Appl. Environ. Microbiol. 66: 3784-3789 [Abstract] [Full Text]  
• Sayyed, A. H., Haward, R., Herrero, S., Ferré, J., Wright, D. J. (2000). Genetic and Biochemical Approach for Characterization of Resistance to Bacillus thuringiensis Toxin Cry1Ac in a Field Population of the Diamondback Moth, Plutella xylostella. Appl. Environ. Microbiol. 66: 1509-1516 [Abstract] [Full Text]  
• Ballester, V., Granero, F., Tabashnik, B. E., Malvar, T., Ferré, J. (1999). Integrative Model for Binding of Bacillus thuringiensis Toxins in Susceptible and Resistant Larvae of the Diamondback Moth (Plutella xylostella). Appl. Environ. Microbiol. 65: 1413-1419 [Abstract] [Full Text]  
• Wirth, M. C., Delécluse, A., Federici, B. A., Walton, W. E. (1998). Variable Cross-Resistance to Cry11B from Bacillus thuringiensis subsp. jegathesan in Culex quinquefasciatus (Diptera: Culicidae) Resistant to Single or Multiple Toxins of Bacillus thuringienisis subsp. israelensis. Appl. Environ. Microbiol. 64: 4174-4179 [Abstract] [Full Text]  
• Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D. R., Dean, D. H. (1998). Bacillus thuringiensis and Its Pesticidal Crystal Proteins. Microbiol. Mol. Biol. Rev. 62: 775-806 [Abstract] [Full Text]  
• Liu, Y.-B., Tabashnik, B. E., Moar, W. J., Smith, R. A. (1998). Synergism between Bacillus thuringiensis Spores and Toxins against Resistant and Susceptible Diamondback Moths (Plutella xylostella). Appl. Environ. Microbiol. 64: 1385-1389 [Abstract] [Full Text]  
• Tabashnik, B. E., Liu, Y.-B., Malvar, T., Heckel, D. G., Masson, L., Ballester, V., Granero, F., Mensua, J. L., Ferre, J. (1997). Global variation in the genetic and biochemical basis of diamondback moth resistance to thuringiensis. Proc. Natl. Acad. Sci. USA 94: 12780-12785 [Abstract] [Full Text]  
• Wirth, M. C., Georghiou, G. P., Federici, B. A. (1997). CytA enables CryIV endotoxins of Bacillus thuringiensis to overcome high levels of CryIV resistance in the mosquito, Culex quinquefasciatus. Proc. Natl. Acad. Sci. USA 94: 10536-10540 [Abstract] [Full Text]  
• Oppert, B., Kramer, K. J., Beeman, R. W., Johnson, D., McGaughey, W. H. (1997). Proteinase-mediated Insect Resistance to Bacillus thuringiensis Toxins. J. Biol. Chem. 272: 23473-23476 [Abstract] [Full Text]  
• Tabashnik, B. E., Liu, Y.-B., Finson, N., Masson, L., Heckel, D. G. (1997). One gene in diamondback moth confers resistance to four Bacillus thuringiensis toxins. Proc. Natl. Acad. Sci. USA 94: 1640-1644 [Abstract] [Full Text]