This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sharma, P. K. Articles by McCarty, P. L. Search for Related Content PubMed PubMed Citation Articles by Sharma, P. K. Articles by McCarty, P. L. Agricola Articles by Sharma, P. K. Articles by McCarty, P. L.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Mar 1996, 761-765, Vol 62, No. 3
Copyright © 1996, American Society for Microbiology

Isolation and Characterization of a Facultatively Aerobic Bacterium That Reductively Dehalogenates Tetrachloroethene to cis-1,2-Dichloroethene

PK Sharma and PL McCarty
Western Region Hazardous Substance Research Center, Department of Civil Engineering, Stanford University, Stanford, California 94305-4020

A rapidly-growing facultatively aerobic bacterium that transforms tetrachloroethene (PCE) via trichloroethene (TCE) to cis-1,2-dichloroethene (cis-1,2-DCE) at high rates in a defined medium was isolated from a contaminated site. Metabolic characterization, cellular fatty acid analysis, and partial sequence analysis of 16S rRNA showed that the new isolate, strain MS-1, has characteristics matching those of the members of the family Enterobacteriaceae. Strain MS-1 can oxidize about 58 substrates including many carbohydrates, short-chain fatty acids, amino acids, purines, and pyrimidines. It can transform up to 1 mM PCE (aqueous) at a rate of about 0.5 (mu)mol of PCE(middot) h(sup-1)(middot)mg (dry weight) of cell(sup-1). PCE transformation occurs following growth on or with the addition of single carbon sources such as glucose, pyruvate, formate, lactate, or acetate or with complex nutrient sources such as yeast extract or a mixture of amino acids. PCE dehalogenation requires the absence of oxygen, nitrate, and high concentrations of fermentable compounds such as glucose. Enterobacter agglomerans biogroup 5 (ATCC 27993), a known facultative bacterium that is closely related to strain MS-1, also reductively dehalogenated PCE to cis-1,2-DCE. To our knowledge, this is the first report on isolation of a facultative bacterium that can reductively transform PCE to cis-1,2-DCE under defined physiological conditions. Also, this is the first report of the ability of E. agglomerans to dehalogenate PCE.

This article has been cited by other articles:

• West, K. A., Johnson, D. R., Hu, P., DeSantis, T. Z., Brodie, E. L., Lee, P. K. H., Feil, H., Andersen, G. L., Zinder, S. H., Alvarez-Cohen, L. (2008). Comparative Genomics of "Dehalococcoides ethenogenes" 195 and an Enrichment Culture Containing Unsequenced "Dehalococcoides" Strains. Appl. Environ. Microbiol. 74: 3533-3540 [Abstract] [Full Text]  
• He, J., Holmes, V. F., Lee, P. K. H., Alvarez-Cohen, L. (2007). Influence of Vitamin B12 and Cocultures on the Growth of Dehalococcoides Isolates in Defined Medium. Appl. Environ. Microbiol. 73: 2847-2853 [Abstract] [Full Text]  
• Ritalahti, K. M., Amos, B. K., Sung, Y., Wu, Q., Koenigsberg, S. S., Loffler, F. E. (2006). Quantitative PCR Targeting 16S rRNA and Reductive Dehalogenase Genes Simultaneously Monitors Multiple Dehalococcoides Strains. Appl. Environ. Microbiol. 72: 2765-2774 [Abstract] [Full Text]  
• Macbeth, T. W., Cummings, D. E., Spring, S., Petzke, L. M., Sorenson, K. S. Jr. (2004). Molecular Characterization of a Dechlorinating Community Resulting from In Situ Biostimulation in a Trichloroethene-Contaminated Deep, Fractured Basalt Aquifer and Comparison to a Derivative Laboratory Culture. Appl. Environ. Microbiol. 70: 7329-7341 [Abstract] [Full Text]  
• Maillard, J., Schumacher, W., Vazquez, F., Regeard, C., Hagen, W. R., Holliger, C. (2003). Characterization of the Corrinoid Iron-Sulfur Protein Tetrachloroethene Reductive Dehalogenase of Dehalobacter restrictus. Appl. Environ. Microbiol. 69: 4628-4638 [Abstract] [Full Text]  
• Sung, Y., Ritalahti, K. M., Sanford, R. A., Urbance, J. W., Flynn, S. J., Tiedje, J. M., Loffler, F. E. (2003). Characterization of Two Tetrachloroethene-Reducing, Acetate-Oxidizing Anaerobic Bacteria and Their Description as Desulfuromonas michiganensis sp. nov.. Appl. Environ. Microbiol. 69: 2964-2974 [Abstract] [Full Text]  
• He, J., Ritalahti, K. M., Aiello, M. R., Loffler, F. E. (2003). Complete Detoxification of Vinyl Chloride by an Anaerobic Enrichment Culture and Identification of the Reductively Dechlorinating Population as a Dehalococcoides Species. Appl. Environ. Microbiol. 69: 996-1003 [Abstract] [Full Text]  
• Rossetti, S., Blackall, L. L., Majone, M., Hugenholtz, P., Plumb, J. J., Tandoi, V. (2003). Kinetic and phylogenetic characterization of an anaerobic dechlorinating microbial community. Microbiology 149: 459-469 [Abstract] [Full Text]  
• Canada, K. A., Iwashita, S., Shim, H., Wood, T. K. (2002). Directed Evolution of Toluene ortho-Monooxygenase for Enhanced 1-Naphthol Synthesis and Chlorinated Ethene Degradation. J. Bacteriol. 184: 344-349 [Abstract] [Full Text]  
• Maymó-Gatell, X., Anguish, T., Zinder, S. H. (1999). Reductive Dechlorination of Chlorinated Ethenes and 1,2-Dichloroethane by "Dehalococcoides ethenogenes" 195. Appl. Environ. Microbiol. 65: 3108-3113 [Abstract] [Full Text]  
• Kengen, S. W. M., Breidenbach, C. G., Felske, A., Stams, A. J. M., Schraa, G., de Vos, W. M. (1999). Reductive Dechlorination of Tetrachloroethene to cis-1,2-Dichloroethene by a Thermophilic Anaerobic Enrichment Culture. Appl. Environ. Microbiol. 65: 2312-2316 [Abstract] [Full Text]