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Appl. Environ. Microbiol., 04 1996, 1448-1451, Vol 62, No. 4
Copyright © 1996, American Society for Microbiology

3-Hydroxypropionaldehyde, an inhibitory metabolite of glycerol fermentation to 1,3-propanediol by enterobacterial species

F Barbirato, JP Grivet, P Soucaille and A Bories
Laboratoire de Biotechnologie de l'Environnement, Institut National de la Recherche Agronomique, Narbonne, France.

Glycerol fermentation by Enterobacter agglomerans revealed that both growth and 1,3-propanediol production ceased after consumption of about 430 mM glycerol, irrespective of the initial glycerol content. This phenomenon was assigned to the production of 3-hydroxypropionaldehyde, which was identified by proton nuclear magnetic resonance and which showed a bacteriostatic effect. The accumulation during glycerol fermentation was also observed with two other enterobacterial species, i.e., Klebsiella pneumoniae and Citrobacter freundii.


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