This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marie, D. Articles by Partensky, F. Search for Related Content PubMed PubMed Citation Articles by Marie, D. Articles by Partensky, F. Agricola Articles by Marie, D. Articles by Partensky, F.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 05 1996, 1649-1655, Vol 62, No. 5
Copyright © 1996, American Society for Microbiology

Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analysis of marine prokaryotes

D Marie, D Vaulot and F Partensky
Centre National de la Recherche Scientifique, Roscoff, France. marie@sb- roscoff.fr

Novel blue light-excited fluorescent dyes for nucleic acids (YOYO-1, YO- PRO-1, and PicoGreen) were tested on cultures of Escherichia coli and of a variety of marine prokaryotes. Results of flow cytometric DNA analyses were compared with those obtained with the UV-excited dyes bis- benzimide Hoechst 33342 or 4', 6-diamidino-2-phenylindole (DAPI). YOYO- 1, YO-PRO-1, and PicoGreen can be used only on aldehyde-fixed cells and need to be supplemented with cofactors such as potassium, citrate, or EDTA. They are highly sensitive to ionic strength. Consequently, seawater culture samples cannot be stained directly with these dyes and require at least a 10-fold dilution with distilled water to obtain reliable fluorescence signals. After treatment with RNase, coefficients of variation for the G1 peak of the DNA distributions of the different strains tested with YOYO-1 or PicoGreen indicated in general an improvement over Hoechst 33342 staining. These novel dyes can be used to enumerate prokaryotic cells by flow cytometry, as demonstrated with E. coli. However, their sensitivity to ionic strength makes them unsuitable for cell cycle analysis in natural samples.

This article has been cited by other articles:

• Corellou, F., Camasses, A., Ligat, L., Peaucellier, G., Bouget, F.-Y. (2005). Atypical Regulation of a Green Lineage-Specific B-Type Cyclin-Dependent Kinase. Plant Physiol. 138: 1627-1636 [Abstract] [Full Text]  
• Blinkova, A., Hermandson, M. J., Walker, J. R. (2003). Suppression of Temperature-Sensitive Chromosome Replication of an Escherichia coli dnaX(Ts) Mutant by Reduction of Initiation Efficiency. J. Bacteriol. 185: 3583-3595 [Abstract] [Full Text]  
• Zipper, H., Buta, C., Lammle, K., Brunner, H., Bernhagen, J., Vitzthum, F. (2003). Mechanisms underlying the impact of humic acids on DNA quantification by SYBR Green I and consequences for the analysis of soils and aquatic sediments. Nucleic Acids Res 31: e39-e39 [Abstract] [Full Text]  
• Graff, A., Sauer, M., Van Gelder, P., Meier, W. (2002). Supramolecular Chemistry And Self-assembly Special Feature: Virus-assisted loading of polymer nanocontainer. Proc. Natl. Acad. Sci. USA 99: 5064-5068 [Abstract] [Full Text]  
• Button, D. K., Robertson, B. R. (2001). Determination of DNA Content of Aquatic Bacteria by Flow Cytometry. Appl. Environ. Microbiol. 67: 1636-1645 [Abstract] [Full Text]  
• Mortimer, F. C., Mason, D. J., Gant, V. A. (2000). Flow Cytometric Monitoring of Antibiotic-Induced Injury in Escherichia coli Using Cell-Impermeant Fluorescent Probes. Antimicrob. Agents Chemother. 44: 676-681 [Abstract] [Full Text]  
• Gasol, J. M., Zweifel, U. L., Peters, F., Fuhrman, J. A., Hagström, A. (1999). Significance of Size and Nucleic Acid Content Heterogeneity as Measured by Flow Cytometry in Natural Planktonic Bacteria. Appl. Environ. Microbiol. 65: 4475-4483 [Abstract] [Full Text]  
• Malandrin, L., Huber, H., Bernander, R. (1999). Nucleoid Structure and Partition in Methanococcus jannaschii: An Archaeon With Multiple Copies of the Chromosome. Genetics 152: 1315-1323 [Abstract] [Full Text]  
• Sieracki, M. E., Cucci, T. L., Nicinski, J. (1999). Flow Cytometric Analysis of 5-Cyano-2,3-Ditolyl Tetrazolium Chloride Activity of Marine Bacterioplankton in Dilution Cultures. Appl. Environ. Microbiol. 65: 2409-2417 [Abstract] [Full Text]  
• Marie, D., Brussaard, C. P. D., Thyrhaug, R., Bratbak, G., Vaulot, D. (1999). Enumeration of Marine Viruses in Culture and Natural Samples by Flow Cytometry. Appl. Environ. Microbiol. 65: 45-52 [Abstract] [Full Text]  
• Weinbauer, M. G., Beckmann, C., Höfle, M. G. (1998). Utility of Green Fluorescent Nucleic Acid Dyes and Aluminum Oxide Membrane Filters for Rapid Epifluorescence Enumeration of Soil and Sediment Bacteria. Appl. Environ. Microbiol. 64: 5000-5003 [Abstract] [Full Text]  
• Lebaron, P., Parthuisot, N., Catala, P. (1998). Comparison of Blue Nucleic Acid Dyes for Flow Cytometric Enumeration of Bacteria in Aquatic Systems. Appl. Environ. Microbiol. 64: 1725-1730 [Abstract] [Full Text]  
• Locher, K. P., Rosenbusch, J. P. (1997). Modeling Ligand-gated Receptor Activity. FhuA-MEDIATED FERRICHROME EFFLUX FROM LIPID VESICLES TRIGGERED BY PHAGE T5. J. Biol. Chem. 272: 1448-1451 [Abstract] [Full Text]  
• Graff, A., Sauer, M., Van Gelder, P., Meier, W. (2002). Supramolecular Chemistry And Self-assembly Special Feature: Virus-assisted loading of polymer nanocontainer. Proc. Natl. Acad. Sci. USA 99: 5064-5068 [Abstract] [Full Text]