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Appl. Environ. Microbiol., Jul 1996, 2216-2220, Vol 62, No. 7
Copyright © 1996, American Society for Microbiology

Coupling of Metabolism and Bioconversion: Microbial Esterification of Citronellol with Acetyl Coenzyme A Produced via Metabolism of Glucose in an Interface Bioreactor

S Oda, Y Inada, A Kobayashi, A Kato, N Matsudomi and H Ohta
Technical Research Laboratory, Kansai Paint Co., Ltd., 4-17-1, Higashi-Yawata, Hiratsuka, Kanagawa 254, Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1, Yoshida, Yamaguchi, Yamaguchi 753, and Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223, Japan

Microbial esterification of citronellol with acetyl coenzyme A (acetyl-CoA) produced via metabolism of glucose was performed with Hansenula saturnus IFO 0809 in an interface bioreactor by using a hydrophilic carrier (an agar plate or an agar-coated filter pad) and a hydrophobic organic solvent (decane). An increase in the glucose concentration on an agar plate led to a decrease in oxidation of citronellol and an increase in the amount of citronellyl acetate. Fed-batch addition of citronellol efficiently alleviated substrate toxicity and resulted in the accumulation of high levels of citronellyl acetate. Furthermore, triple coupling of acetyl-CoA formation, microbial reduction of citronellal to citronellol, and esterification of the resulting citronellol with acetyl-CoA was also an efficient way to prepare citronellyl acetate. By using coupling systems, citronellyl acetate could be efficiently produced without any acetyl donor.

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