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Appl. Environ. Microbiol., Jul 1996, 2644-2646, Vol 62, No. 7
Copyright © 1996, American Society for Microbiology

Effects of atrazine on Ochrobactrum anthropi membrane fatty acids

D Laura, G De Socio, R Frassanito and D Rotilio
Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, "Gennaro Paone" Environmental Health Center, Santa Maria Imbaro, Italy.

Ochrobactrum anthropi is a gram-negative bacillus recognized as a human opportunist pathogen isolated in clinical specimens and not of clinical significance. We report a new aspect of this bacterium, that it has been isolated from activated sludge. In fact, it is able to grow on atrazine (2-chloro-4-ethylamino-6-isopropyl-amine-s-triazine) by utilizing it as the only source of carbon. Our results show that atrazine (0.03 g/liter) causes a dramatical increase in the degree of saturation of membrane fatty acids. Analysis and identification of bacterial fatty acids were performed by gas chromatography and gas chromatography-mass spectrometry techniques.


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