This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Galperin, M. Y. Articles by Romano, A. H. Search for Related Content PubMed PubMed Citation Articles by Galperin, M. Y. Articles by Romano, A. H. Agricola Articles by Galperin, M. Y. Articles by Romano, A. H.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Aug 1996, 2915-2918, Vol 62, No. 8
Copyright © 1996, American Society for Microbiology

The glucose transport system of the hyperthermophilic anaerobic bacterium Thermotoga neapolitana

MY Galperin, KM Noll and AH Romano
Department of Molecular and Cell Biology, The University of Connecticut, Storrs, Connecticut 06269-3125

The glucose transport system of the extremely thermophilic anaerobic bacterium Thermotoga neapolitana was studied with the nonmetabolizable glucose analog 2-deoxy-D-glucose (2-DOG). T. neapolitana accumulated 2- DOG against a concentration gradient in an intracellular free sugar pool that was exchangeable with external source of energy, such as pyruvate, and was inhibited by arsenate and gramicidin D. There was no phosphoenolpyruvate-dependent phosphorylation of glucose, 2-DOG, or fructose by cell extracts or toluene-treated cells, indicating the absence of a phosphoenolpyruvate:sugar phosphotransferase system. These data indicate that D-glucose is taken up by T. neapolitana via an active transport system that is energized by an ion gradient generated by ATP, derived from substrate-level phosphorylation.

This article has been cited by other articles:

• Jorge, C. D., Fonseca, L. L., Boos, W., Santos, H. (2008). Role of Periplasmic Trehalase in Uptake of Trehalose by the Thermophilic Bacterium Rhodothermus marinus. J. Bacteriol. 190: 1871-1878 [Abstract] [Full Text]  
• Chevance, F. F. V., Erhardt, M., Lengsfeld, C., Lee, S.-J., Boos, W. (2006). Mlc of Thermus thermophilus: a Glucose-Specific Regulator for a Glucose/Mannose ABC Transporter in the Absence of the Phosphotransferase System.. J. Bacteriol. 188: 6561-6571 [Abstract] [Full Text]  
• Nguyen, T. N., Ejaz, A. D., Brancieri, M. A., Mikula, A. M., Nelson, K. E., Gill, S. R., Noll, K. M. (2004). Whole-Genome Expression Profiling of Thermotoga maritima in Response to Growth on Sugars in a Chemostat. J. Bacteriol. 186: 4824-4828 [Abstract] [Full Text]  
• Chhabra, S. R., Shockley, K. R., Conners, S. B., Scott, K. L., Wolfinger, R. D., Kelly, R. M. (2003). Carbohydrate-induced Differential Gene Expression Patterns in the Hyperthermophilic Bacterium Thermotoga maritima. J. Biol. Chem. 278: 7540-7552 [Abstract] [Full Text]  
• Dzioba, J., Hase, C. C., Gosink, K., Galperin, M. Y., Dibrov, P. (2003). Experimental Verification of a Sequence-Based Prediction: F1F0-Type ATPase of Vibrio cholerae Transports Protons, Not Na+ Ions. J. Bacteriol. 185: 674-678 [Abstract] [Full Text]  
• Nanavati, D., Noll, K. M., Romano, A. H. (2002). Periplasmic maltose- and glucose-binding protein activities in cell-free extracts of Thermotoga maritima. Microbiology 148: 3531-3537 [Abstract] [Full Text]