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Appl. Environ. Microbiol., Sep 1996, 3466-3469, Vol 62, No. 9
Copyright © 1996, American Society for Microbiology

Prevalence of enterohemorrhagic Escherichia coli in raw and treated municipal sewage

SB Grant, CP Pendroy, CL Mayer, JK Bellin and CJ Palmer
Department of Civil and Environmental Engineering, University of California, Irvine 92717, USA. sbgrant@uci.edu

Municipal sewage was screened for DNA encoding Shiga-like Toxin (SLT) II, a key protein involved in the virulence of enterohemorrhagic Escherichia coli. PCR analysis of sewage concentrates showed that DNA encoding SLT II was present in a single sample of untreated sewage and absent in all other samples tested (n = 6). Thermotolerant E. coli cultured from the sewage (n = 1,520) also tested negative for SLT II by colony hybridization.

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