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Appl. Environ. Microbiol., Sep 1996, 3489-3493, Vol 62, No. 9
Copyright © 1996, American Society for Microbiology

Use of repetitive intergenic DNA sequences to classify pathogenic and disease-suppressive Streptomyces strains

MJ Sadowsky, LL Kinkel, JH Bowers and JL Schottel
Department of Soil, Water, and Climate, University of Minnesota, St. Paul 55108, USA.

PCR DNA fingerprinting using repetitive intergenic DNA sequences (rep- PCR) was investigated as a means of differentiating between closely related strains of Streptomyces which were, in some cases, indistinguishable by other classification methods. Our results demonstrated that the majority of strains had unique rep-PCR DNA fingerprints and established that the technique could be a very useful tool in rapidly determining strain identity.


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