This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chang, I. S. Articles by Shin, P. K. Search for Related Content PubMed PubMed Citation Articles by Chang, I. S. Articles by Shin, P. K. Agricola Articles by Chang, I. S. Articles by Shin, P. K.

Next Article 

Appl. Environ. Microbiol., Jan 1997, 1-6, Vol 63, No. 1
Copyright © 1997, American Society for Microbiology

Use of sulfite and hydrogen peroxide to control bacterial contamination in ethanol fermentation

IS Chang, BH Kim and PK Shin
Environment Research Center, Korea Institute of Science and Technology, Seoul, Korea.

Lactic acid bacteria isolated from an industrial-scale ethanol fermentation process were used to evaluate sulfite as a bacterial- contamination control agent in a cell-recycled continuous ethanol fermentation process. The viabilities of bacteria were decreased by sulfite at concentrations of 100 to 400 mg liter-1, while sulfite at the same concentrations did not change the viability of the Saccharomyces cerevisiae strain used in this process. Sulfite was effective only in the presence of oxygen. Bacteria showed differences in their susceptibilities to sulfite. Facultatively heterofermentative Lactobacillus casei 4-3 was more susceptible than was obligatory heterofermentative Lactobacillus fermentum 7-1. The former showed higher enzyme activities involved in the production and consumption of hydrogen peroxide than did the latter. The viability of L. fermentum 7- 1 could be selectively controlled by hydrogen peroxide at concentrations of 1 to 10 mM. Based on these findings, it is hypothesized that the sulfur trioxide radical anions formed by peroxidase in the presence of hydrogen peroxide are responsible for the control of contaminating bacteria. Sulfite did not kill the yeast strain, which has catalase to degrade hydrogen peroxide. A cell- recycled continuous ethanol fermentation process was run successfully with sulfite treatments.

This article has been cited by other articles:

• Jurat-Fuentes, J. L., Gould, F. L., Adang, M. J. (2003). Dual Resistance to Bacillus thuringiensis Cry1Ac and Cry2Aa Toxins in Heliothis virescens Suggests Multiple Mechanisms of Resistance. Appl. Environ. Microbiol. 69: 5898-5906 [Abstract] [Full Text]  
• Narendranath, N. V., Thomas, K. C., Ingledew, W. M. (2000). Urea Hydrogen Peroxide Reduces the Numbers of Lactobacilli, Nourishes Yeast, and Leaves No Residues in the Ethanol Fermentation. Appl. Environ. Microbiol. 66: 4187-4192 [Abstract] [Full Text]  
• Lodder, W. J., Vinjé, J., van de Heide, R., de Roda Husman, A. M., Leenen, E. J. T. M., Koopmans, M. P. G. (1999). Molecular Detection of Norwalk-Like Caliciviruses in Sewage. Appl. Environ. Microbiol. 65: 5624-5627 [Abstract] [Full Text]