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Appl. Environ. Microbiol., Oct 1997, 3992-3994, Vol 63, No. 10
Copyright © 1997, American Society for Microbiology

Ribotyping for strain characterization of Clostridium perfringens isolates from food poisoning cases and outbreaks

B Schalch, J Bjorkroth, H Eisgruber, H Korkeala and A Stolle
Institute for Hygiene and Technology of Foods of Animal Origin, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany.

Ribotyping was used to characterize 34 Clostridium perfringens strains isolated from 10 food poisoning cases and outbreaks over a 7-year period. Twelve different ribopatterns were generated by EcoRI digestion. In eight food poisoning cases and outbreaks, all of the ribotypes of each food and stool isolate were found to be identical. Two C. perfringens isolates showed unique patterns. Ribotyping was found to be a useful tool for determining the genetic relationship of C. perfringens isolates in the context of foodborne poisoning cases.

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