This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ramos, A.
Right arrow Articles by Santos, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramos, A.
Right arrow Articles by Santos, H.
Agricola
Right arrow Articles by Ramos, A.
Right arrow Articles by Santos, H.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Oct 1997, 4020-4025, Vol 63, No. 10
Copyright © 1997, American Society for Microbiology

Stabilization of Enzymes against Thermal Stress and Freeze-Drying by Mannosylglycerate

A Ramos, NDH Raven, RJ Sharp, S Bartolucci, M Rossi, R Cannio, J Lebbink, J Van Der Oost, WM De Vos and H Santos
Instituto de Tecnologia Quimica e Biologica, ITQB/UNL, Oeiras, Portugal; Centre for Applied Microbiology and Research, Porton Down, Salisbury, United Kingdom; Dip. Chimica Organica e Biologica, Universita di Napoli, Napoli, Italy; and Department of Microbiology, Agricultural University of Wageningen, Wageningen, The Netherlands

2-O-(beta)-Mannosylglycerate, a solute that accumulates in some (hyper)thermophilic organisms, was purified from Pyrococcus furiosus cells, and its effect on enzyme stabilization in vitro was assessed. Enzymes from hyperthermophilic, thermophilic, and mesophilic sources were examined. The thermostabilities of alcohol dehydrogenases from P. furiosus and Bacillus stearothermophilus and of glutamate dehydrogenases from Thermotoga maritima and Clostridium difficile were improved to a significant extent when enzyme solutions were incubated at supraoptimal temperatures in the presence of 2-O-(beta)-mannosylglycerate, but no effect on the thermostability of glutamate dehydrogenase from P. furiosus was detected. On the other hand, there was a remarkable effect on the thermal stabilities of rabbit muscle lactate dehydrogenase, baker's yeast alcohol dehydrogenase, and bovine liver glutamate dehydrogenase, which were used as model systems to evaluate stabilization of enzymes of mesophilic origin. For all of the enzymes examined and at the highest temperatures tested, 2-O-(beta)-mannosylglycerate was a better thermoprotectant than trehalose. The stabilizing effect exerted by 2-O-(beta)-mannosylglycerate on enzymes suggests a role for this compound as a protein thermostabilizer under physiological conditions. 2-O-(beta)-Mannosylglycerate was also effective in the protection of enzymes against stress imposed by freeze-drying, with its protecting effect being similar to or better than that exerted by trehalose. The data show 2-O-(beta)-mannosylglycerate to be a potential enzyme stabilizer in biotechnological applications.


This article has been cited by other articles:

  • Bursy, J., Kuhlmann, A. U., Pittelkow, M., Hartmann, H., Jebbar, M., Pierik, A. J., Bremer, E. (2008). Synthesis and Uptake of the Compatible Solutes Ectoine and 5-Hydroxyectoine by Streptomyces coelicolor A3(2) in Response to Salt and Heat Stresses. Appl. Environ. Microbiol. 74: 7286-7296 [Abstract] [Full Text]  
  • Yang, Y.-L., Yang, F.-L., Jao, S.-C., Chen, M.-Y., Tsay, S.-S., Zou, W., Wu, S.-H. (2006). Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus. J. Lipid Res. 47: 1823-1832 [Abstract] [Full Text]  
  • Neves, C., da Costa, M. S., Santos, H. (2005). Compatible Solutes of the Hyperthermophile Palaeococcus ferrophilus: Osmoadaptation and Thermoadaptation in the Order Thermococcales. Appl. Environ. Microbiol. 71: 8091-8098 [Abstract] [Full Text]  
  • Faria, T. Q., Lima, J. C., Bastos, M., Macanita, A. L., Santos, H. (2004). Protein Stabilization by Osmolytes from Hyperthermophiles: EFFECT OF MANNOSYLGLYCERATE ON THE THERMAL UNFOLDING OF RECOMBINANT NUCLEASE A FROM STAPHYLOCOCCUS AUREUS STUDIED BY PICOSECOND TIME-RESOLVED FLUORESCENCE AND CALORIMETRY. J. Biol. Chem. 279: 48680-48691 [Abstract] [Full Text]  
  • Empadinhas, N., Albuquerque, L., Costa, J., Zinder, S. H., Santos, M. A. S., Santos, H., da Costa, M. S. (2004). A Gene from the Mesophilic Bacterium Dehalococcoides ethenogenes Encodes a Novel Mannosylglycerate Synthase. J. Bacteriol. 186: 4075-4084 [Abstract] [Full Text]  
  • Sampaio, M.-M., Chevance, F., Dippel, R., Eppler, T., Schlegel, A., Boos, W., Lu, Y.-J., Rock, C. O. (2004). Phosphotransferase-mediated Transport of the Osmolyte 2-O-{alpha}-Mannosyl-D-glycerate in Escherichia coli Occurs by the Product of the mngA (hrsA) Gene and Is Regulated by the mngR (farR) Gene Product Acting as Repressor. J. Biol. Chem. 279: 5537-5548 [Abstract] [Full Text]  
  • Empadinhas, N., Albuquerque, L., Henne, A., Santos, H., Costa, M. S. d. (2003). The Bacterium Thermus thermophilus, Like Hyperthermophilic Archaea, Uses a Two-Step Pathway for the Synthesis of Mannosylglycerate. Appl. Environ. Microbiol. 69: 3272-3279 [Abstract] [Full Text]  
  • Sampaio, M.-M., Santos, H., Boos, W. (2003). Synthesis of GDP-Mannose and Mannosylglycerate from Labeled Mannose by Genetically Engineered Escherichia coli without Loss of Specific Isotopic Enrichment. Appl. Environ. Microbiol. 69: 233-240 [Abstract] [Full Text]  
  • Empadinhas, N., Marugg, J. D., Borges, N., Santos, H., da Costa, M. S. (2001). Pathway for the Synthesis of Mannosylglycerate in the Hyperthermophilic Archaeon Pyrococcus horikoshii. BIOCHEMICAL AND GENETIC CHARACTERIZATION OF KEY ENZYMES. J. Biol. Chem. 276: 43580-43588 [Abstract] [Full Text]  
  • Kelty, J., Lee, R. Jr (2001). Rapid cold-hardening of Drosophila melanogaster (Diptera: Drosophiladae) during ecologically based thermoperiodic cycles. J. Exp. Biol. 204: 1659-1666 [Abstract]  
  • Lamosa, P., Burke, A., Peist, R., Huber, R., Liu, M.-Y., Silva, G., Rodrigues-Pousada, C., LeGall, J., Maycock, C., Santos, H. (2000). Thermostabilization of Proteins by Diglycerol Phosphate, a New Compatible Solute from the Hyperthermophile Archaeoglobus fulgidus. Appl. Environ. Microbiol. 66: 1974-1979 [Abstract] [Full Text]  
  • Martins, L. O., Empadinhas, N., Marugg, J. D., Miguel, C., Ferreira, C., da Costa, M. S., Santos, H. (1999). Biosynthesis of Mannosylglycerate in the Thermophilic Bacterium Rhodothermus marinus. BIOCHEMICAL AND GENETIC CHARACTERIZATION OF A MANNOSYLGLYCERATE SYNTHASE. J. Biol. Chem. 274: 35407-35414 [Abstract] [Full Text]  
  • Cánovas, D., Borges, N., Vargas, C., Ventosa, A., Nieto, J. J., Santos, H. (1999). Role of Ngamma -Acetyldiaminobutyrate as an Enzyme Stabilizer and an Intermediate in the Biosynthesis of Hydroxyectoine. Appl. Environ. Microbiol. 65: 3774-3779 [Abstract] [Full Text]  
  • Matussek, K., Moritz, P., Brunner, N., Eckerskorn, C., Hensel, R. (1998). Cloning, Sequencing, and Expression of the Gene Encoding Cyclic 2,3-Diphosphoglycerate Synthetase, the Key Enzyme of Cyclic 2,3-Diphosphoglycerate Metabolism in Methanothermus fervidus. J. Bacteriol. 180: 5997-6004 [Abstract] [Full Text]  
  • Lamosa, P., Martins, L. O., Da Costa, M. S., Santos, H. (1998). Effects of Temperature, Salinity, and Medium Composition on Compatible Solute Accumulation by Thermococcus spp.. Appl. Environ. Microbiol. 64: 3591-3598 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»